Leibniz-Institut für Agrartechnik Potsdam-Bornim e.V. (ATB), Abteilung Bioverfahrenstechnik, Potsdam, Germany.
FEMS Microbiol Ecol. 2012 Mar;79(3):785-99. doi: 10.1111/j.1574-6941.2011.01265.x. Epub 2011 Dec 20.
DNAs of two biofilms of a thermophilic two-phase leach-bed biogas reactor fed with rye silage and winter barley straw were sequenced by 454-pyrosequencing technology to assess the biofilm-based microbial community and their genetic potential for anaerobic digestion. The studied biofilms matured on the surface of the substrates in the hydrolysis reactor (HR) and on the packing in the anaerobic filter reactor (AF). The classification of metagenome reads showed Clostridium as most prevalent bacteria in the HR, indicating a predominant role for plant material digestion. Notably, insights into the genetic potential of plant-degrading bacteria were determined as well as further bacterial groups, which may assist Clostridium in carbohydrate degradation. Methanosarcina and Methanothermobacter were determined as most prevalent methanogenic archaea. In consequence, the biofilm-based methanogenesis in this system might be driven by the hydrogenotrophic pathway but also by the aceticlastic methanogenesis depending on metabolite concentrations such as the acetic acid concentration. Moreover, bacteria, which are capable of acetate oxidation in syntrophic interaction with methanogens, were also predicted. Finally, the metagenome analysis unveiled a large number of reads with unidentified microbial origin, indicating that the anaerobic degradation process may also be conducted by up to now unknown species.
采用 454 焦磷酸测序技术对以黑麦青贮和冬大麦秸秆为原料的嗜热两相渗滤床沼气发酵生物膜的两个生物膜样本的 DNA 进行测序，以评估基于生物膜的微生物群落及其在厌氧消化中的遗传潜力。所研究的生物膜在水解反应器（HR）的基质表面和厌氧滤池反应器（AF）的填料上成熟。宏基因组reads 的分类表明，HR 中最常见的细菌是梭菌，这表明其在植物材料消化中起主要作用。值得注意的是，还确定了植物降解细菌的遗传潜力以及其他可能有助于梭菌进行碳水化合物降解的细菌群体。产甲烷菌和产甲烷八叠球菌被确定为最常见的产甲烷古菌。因此，该系统基于生物膜的产甲烷作用可能由氢营养型途径驱动，但也可能由乙酸营养型产甲烷作用驱动，具体取决于代谢物浓度，如乙酸浓度。此外，还预测了能够与产甲烷菌在协同作用中氧化乙酸的细菌。最后，宏基因组分析揭示了大量具有未知微生物来源的reads，这表明厌氧降解过程可能也由迄今未知的物种进行。
