Section of Periodontics, School of Dentistry, University of California, Los Angeles, CA 90095, USA.
J Periodontal Res. 2012 Aug;47(4):409-17. doi: 10.1111/j.1600-0765.2011.01446.x. Epub 2011 Nov 29.
Bovine porous bone mineral (BPBM) is a xenograft that has been successfully utilized in periodontal regeneration. Platelet-rich fibrin (PRF) is a leukocyte and platelet preparation that concentrates various polypeptide growth factors and therefore has the potential to be used as regenerative treatment for periodontal defects. The purpose of this study was to examine the suitability of autologous PRF as regenerative treatment for periodontal intrabony defects in humans and to examine the ability of BPBM to augment the regenerative effects exerted by PRF.
Using a split-mouth design, 17 paired intrabony defects were randomly treated either with PRF or with PRF-BPBM combination. Re-entry surgeries were performed at 6 mo. Primary study outcomes were changes in pocket depth, attachment level and defect fill.
Preoperative pocket depths, attachment levels and transoperative bone measurements were similar for the PRF and PRF-BPBM groups. Postsurgical measurements revealed a significantly greater reduction in pocket depth in the PRF-BPBM group (4.47±0.78 mm on buccal and 4.29±0.82 mm on lingual sites) when compared with the PRF group (3.35±0.68 mm on buccal and 3.24±0.73 mm on lingual sites). The PRF-BPBM group presented with significantly greater attachment gain (3.82±0.78 mm on buccal and 3.71±0.75 mm on lingual sites) than the PRF group (2.24±0.73 mm on buccal and 2.12±0.68 mm on lingual sites). Defect fill was also greater in the PRF-BPBM group (4.06±0.87 mm on buccal and 3.94±0.73 mm on lingual sites) than in the PRF group (2.21±0.68 mm on buccal and 2.06±0.64 mm on lingual sites).
The results of this study indicate that PRF can improve clinical parameters associated with human intrabony periodontal defects, and BPBM has the ability to augment the effects of PRF in reducing pocket depth, improving clinical attachment levels and promoting defect fill.
牛多孔骨矿物质（BPBM）是一种已成功应用于牙周再生的异种移植物。富含血小板的纤维蛋白（PRF）是一种白细胞和血小板制剂，浓缩了各种多肽生长因子，因此具有作为牙周缺陷再生治疗的潜力。本研究的目的是检查自体 PRF 作为人类牙周骨内缺损再生治疗的适宜性，并研究 BPBM 增强 PRF 再生效果的能力。
采用分口设计，将 17 对骨内缺损随机分为 PRF 组或 PRF-BPBM 联合组。在 6 个月时进行再进入手术。主要研究结果为牙周袋深度、附着水平和缺损填充的变化。
PRF 和 PRF-BPBM 组的术前牙周袋深度、附着水平和术中骨测量值相似。术后测量显示，PRF-BPBM 组（颊侧 4.47±0.78mm，舌侧 4.29±0.82mm）较 PRF 组（颊侧 3.35±0.68mm，舌侧 3.24±0.73mm）的牙周袋深度显著降低。PRF-BPBM 组的附着水平显著增加（颊侧 3.82±0.78mm，舌侧 3.71±0.75mm），高于 PRF 组（颊侧 2.24±0.73mm，舌侧 2.12±0.68mm）。PRF-BPBM 组的缺损填充也大于 PRF 组（颊侧 4.06±0.87mm，舌侧 3.94±0.73mm）（颊侧 2.21±0.68mm，舌侧 2.06±0.64mm）。
本研究结果表明，PRF 可改善与人类骨内牙周缺损相关的临床参数，BPBM 具有增强 PRF 降低牙周袋深度、改善临床附着水平和促进缺损填充的作用。
