Marine Biotechnology Centre, Environmental Research Institute, University College Cork, Cork, Ireland.
J Appl Microbiol. 2012 Feb;112(2):289-301. doi: 10.1111/j.1365-2672.2011.05211.x. Epub 2011 Dec 22.
To evaluate the diversity and antimicrobial activity of bacteria from the marine sponges Suberites carnosus and Leucosolenia sp.
Two hundred and thirty-seven bacteria were isolated from the sponges S. carnosus (Demospongiae) and Leucosolenia sp. (Calcarea). Isolates from the phyla Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria were obtained. Isolates of the genus Pseudovibrio were dominant among the bacteria from S. carnosus, whereas Pseudoalteromonas and Vibrio were the dominant genera isolated from Leucosolenia sp. Approximately 50% of the isolates from S. carnosus displayed antibacterial activity, and c. 15% of the isolates from Leucosolenia sp. demonstrated activity against the test fungal strains. The antibacterial activity observed was mostly from Pseudovibrio and Spongiobacter isolates, while the majority of the antifungal activity was observed from the Pseudoalteromonas, Bacillus and Vibrio isolates.
Both sponges possess a diverse range of bioactive and potentially novel bacteria. Differences observed from the sponge-derived groups of isolates in terms of bioactivity suggest that S. carnosus isolates may be a better source of antibacterial compounds, while Leucosolenia sp. isolates appear to be a better source of antifungal compounds.
This is the first study in which cultured bacterial isolates from the marine sponges S. carnosus and a Leucosolenia sp. have been evaluated for their antibacterial activity. The high percentage of antibacterial isolates from S. carnosus and of antifungal isolates from Leucosolenia sp. suggests that these two sponges may be good sources for potentially novel marine natural products.
评估海洋海绵 Suberites carnosus 和 Leucosolenia sp. 中细菌的多样性和抗菌活性。
从海绵 Suberites carnosus（Demospongiae）和 Leucosolenia sp.（Calcarea）中分离出 237 株细菌。分离出的菌株分别属于放线菌门、拟杆菌门、厚壁菌门和变形菌门。其中，假单胞菌属和弧菌属是从 S. carnosus 中分离出的优势属，而假交替单胞菌属和发光杆菌属是从 Leucosolenia sp. 中分离出的优势属。来自 S. carnosus 的分离物中约有 50%显示出抗菌活性，来自 Leucosolenia sp. 的分离物中约有 15%对测试真菌菌株具有活性。观察到的抗菌活性主要来自假单胞菌属和海绵杆菌属的分离物，而大多数抗真菌活性则来自假交替单胞菌属、芽孢杆菌属和弧菌属的分离物。
两种海绵都拥有丰富多样的具有生物活性和潜在新颖性的细菌。从海绵衍生的分离物组在生物活性方面观察到的差异表明，S. carnosus 分离物可能是抗菌化合物的更好来源，而 Leucosolenia sp. 分离物似乎是抗真菌化合物的更好来源。
这是首次评估来自海洋海绵 Suberites carnosus 和 Leucosolenia sp. 的培养细菌分离物的抗菌活性。来自 S. carnosus 的高比例抗菌分离物和来自 Leucosolenia sp. 的高比例抗真菌分离物表明，这两种海绵可能是潜在新型海洋天然产物的良好来源。
