蛋白激酶C的激活导致其肉豆蔻酰化、富含丙氨酸的底物从巨噬细胞丝状伪足中的点状结构上移位。
Activation of protein kinase C results in the displacement of its myristoylated, alanine-rich substrate from punctate structures in macrophage filopodia.
作者信息
Rosen A, Keenan K F, Thelen M, Nairn A C, Aderem A
机构信息
Rockefeller University, New York, NY 10021.
出版信息
J Exp Med. 1990 Oct 1;172(4):1211-5. doi: 10.1084/jem.172.4.1211.
Abstract
The myristoylated, alanine-rich C kinase substrate (MARCKS) is a prominent substrate for protein kinase C (PKC) in a variety of cells, and has been implicated in diverse cellular processes including neurosecretion, fibroblast mitogenesis, and macrophage activation. In macrophages that have spread on the substratum, MARCKS has a punctate distribution at the cell-substratum interface of pseudopodia and filopodia. At these points, MARCKS co-localizes with vinculin and talin. Activation of PKC with phorbol esters results in the rapid disappearance of punctate staining of MARCKS, but not vinculin or talin, and is accompanied by cell spreading and loss of filopodia. The morphological changes and disappearance of punctate staining follow a time-course that closely approximates both the PKC-dependent phosphorylation of MARCKS, and its phosphorylation-dependent release from the plasma membrane. Our results suggest a role for PKC-dependent phosphorylation of MARCKS in the regulation of the membrane cytoskeleton.
摘要
豆蔻酰化富含丙氨酸的蛋白激酶C底物（MARCKS）是多种细胞中蛋白激酶C（PKC）的主要底物，并且参与了包括神经分泌、成纤维细胞有丝分裂和巨噬细胞激活在内的多种细胞过程。在铺展于基质上的巨噬细胞中，MARCKS在伪足和丝状伪足的细胞-基质界面呈点状分布。在这些部位，MARCKS与纽蛋白和踝蛋白共定位。用佛波酯激活PKC会导致MARCKS点状染色迅速消失，但纽蛋白或踝蛋白不受影响，同时伴随着细胞铺展和丝状伪足消失。形态学变化和点状染色消失的时间进程与MARCKS依赖PKC的磷酸化及其从质膜的磷酸化依赖性释放密切相关。我们的数据表明，MARCKS依赖PKC的磷酸化在膜细胞骨架的调节中发挥作用。
相似文献
1
Activation of protein kinase C results in the displacement of its myristoylated, alanine-rich substrate from punctate structures in macrophage filopodia.蛋白激酶C的激活导致其肉豆蔻酰化、富含丙氨酸的底物从巨噬细胞丝状伪足中的点状结构上移位。
J Exp Med. 1990 Oct 1;172(4):1211-5. doi: 10.1084/jem.172.4.1211.
2
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3
Activation of protein kinase C-alpha and translocation of the myristoylated alanine-rich C-kinase substrate correlate with phorbol ester-enhanced noradrenaline release from SH-SY5Y human neuroblastoma cells.蛋白激酶C-α的激活以及富含肉豆蔻酰化丙氨酸的C激酶底物的转位与佛波酯增强去甲肾上腺素从SH-SY5Y人神经母细胞瘤细胞中的释放相关。
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4
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5
Importance of protein kinase C targeting for the phosphorylation of its substrate, myristoylated alanine-rich C-kinase substrate.蛋白激酶C靶向作用对其底物豆蔻酰化富含丙氨酸的蛋白激酶C底物磷酸化的重要性。
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Calmodulin and protein kinase C cross-talk: the MARCKS protein is an actin filament and plasma membrane cross-linking protein regulated by protein kinase C phosphorylation and by calmodulin.钙调蛋白与蛋白激酶C的相互作用：MARCKS蛋白是一种肌动蛋白丝和质膜交联蛋白，受蛋白激酶C磷酸化和钙调蛋白调控。
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8
Arginine vasopressin (AVP) causes the reversible phosphorylation of the myristoylated alanine-rich C kinase substrate (MARCKS) protein in the ovine anterior pituitary: evidence that MARCKS phosphorylation is associated with adrenocorticotropin (ACTH) secretion.精氨酸加压素（AVP）可使绵羊垂体前叶中富含肉豆蔻酰化丙氨酸的蛋白激酶C底物（MARCKS）蛋白发生可逆磷酸化：有证据表明，MARCKS磷酸化与促肾上腺皮质激素（ACTH）分泌有关。
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Myristoylated alanine-rich C-kinase substrate is phosphorylated and translocated by a phorbol ester-insensitive and calcium-independent protein kinase C isoform in C6 glioma cell membranes.豆蔻酰化富含丙氨酸的蛋白激酶C底物在C6胶质瘤细胞膜中被一种佛波酯不敏感且不依赖钙的蛋白激酶C亚型磷酸化并发生易位。
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Adhesion plaques: sites of transmembrane interaction between the extracellular matrix and the actin cytoskeleton.黏着斑：细胞外基质与肌动蛋白细胞骨架之间跨膜相互作用的位点。
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