Zwicker Jeffrey I, Lacroix Romaric, Dignat-George Françoise, Furie Barbara C, Furie Bruce
Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Methods Mol Biol. 2012;788:127-39. doi: 10.1007/978-1-61779-307-3_10.
Platelet microparticles are submicron vesicles that can support thrombin generation on externalized negatively charged phospholipids. Increased numbers of circulating platelet microparticles have been investigated as the basis of hypercoagulability in a variety of prothrombotic conditions. Measurement of platelet microparticles is not standardized and a number of preanalytic considerations can influence accurate analysis. We describe methodology for light scatter-based flow cytometry as well as impedance-based flow cytometry for the enumeration and characterization of platelet microparticles.
血小板微粒是亚微米级的囊泡，可在暴露于外部的带负电荷磷脂上支持凝血酶生成。循环血小板微粒数量增加已被研究作为多种血栓前状态下高凝性的基础。血小板微粒的测量尚未标准化，许多分析前的因素会影响准确分析。我们描述了基于光散射的流式细胞术以及基于阻抗的流式细胞术的方法，用于血小板微粒的计数和表征。
