Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.
Semin Thromb Hemost. 2010 Nov;36(8):819-23. doi: 10.1055/s-0030-1267035. Epub 2010 Nov 3.
Over the last several years, there has been considerable interest in evaluating the biological relevance of alterations in blood-borne microparticle populations. The most commonly employed technique for the characterization of microparticles is light scatter flow cytometry. However, the enumeration and sizing of submicron particles based on light scattering properties can be problematic. Impedance-based flow cytometry based on the Coulter principle offers a sensitive methodology to characterize microparticles. This review details the rationale for employing impedance-based flow cytometry in the measurement of blood-borne microparticles.
在过去几年中，人们对评估血液来源的微粒群体变化的生物学相关性产生了浓厚的兴趣。用于微粒特征描述的最常用技术是光散射流式细胞术。然而，基于光散射特性对亚微米颗粒进行计数和定尺寸可能会有问题。基于库尔特原理的阻抗流式细胞术提供了一种敏感的方法来表征微粒。本综述详细介绍了在测量血液来源的微粒时采用基于阻抗的流式细胞术的基本原理。
