Samadi Nasrin, Zaree Reyhane, Bakhtiar Hengameh, Salehnia Alinazar, Azimi Shahram
Assistant Professor, Department of Drug and Food Control, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Dent Res J (Isfahan). 2011 Winter;8(1):28-32.
The aim of this study was to compare the antibacterial efficacy of endemic Satureja Khuzistanica Jamzad (SKJ) essential oil as root canal irrigation versus 2.5% sodium hypochlorite and 2% chlorhexidine gluconate.
In current in vitro experimental study, fifty four single-rooted teeth were randomly divided into 6 groups of 9 samples: 2.5% sodium hypochlorite (NaOCl), 2% chlorhexidine gluconate (CHX), 0.31 mg/ml SKJ, 0.62 mg/ml SKJ, positive and negative controls. Each tooth was instrumented, sealed and autoclaved. Then, test groups were inoculated with E. faecalis, treated with irrigation solution and viable bacterial counts in intracanal dentin chips were determined. Utilizing SPSS 18 software, collected data were analyzed by Kruskal-Wallis one way analysis of variance (P = 0.05).
99.94 % and 99.50% reduction in bacteria load after 5 min treatment with NaOCl and CHX were detected, respectively. Similarly, 99.97% and 99.96% reduction in bacterial counts were observed after 5 min application of 0.62 mg/ml and 0.31 mg/ml SKJ essential. No significant differences were detected among the four irrigation solutions (P = 0.755).
SKJ essential oil with the minimum inhibitory concentration (MIC) of 0.31 mg/ml could be an effective antibacterial irrigation solution.
本研究旨在比较伊朗胡齐斯坦省特有植物皱叶薄荷（SKJ）精油作为根管冲洗剂与2.5%次氯酸钠和2%葡萄糖酸氯己定的抗菌效果。
在当前的体外实验研究中，54颗单根牙被随机分为6组，每组9个样本：2.5%次氯酸钠（NaOCl）、2%葡萄糖酸氯己定（CHX）、0.31 mg/ml SKJ、0.62 mg/ml SKJ、阳性和阴性对照。每颗牙齿经过预备、密封和高压灭菌。然后，测试组接种粪肠球菌，用冲洗液处理，并测定根管内牙本质碎屑中的活菌计数。使用SPSS 18软件，通过Kruskal-Wallis单因素方差分析对收集的数据进行分析（P = 0.05）。
用NaOCl和CHX处理5分钟后，细菌载量分别降低了99.94%和99.50%。同样，在应用0.62 mg/ml和0.31 mg/ml SKJ精油5分钟后，细菌计数分别降低了99.97%和99.96%。四种冲洗液之间未检测到显著差异（P = 0.755）。
最低抑菌浓度（MIC）为0.31 mg/ml的SKJ精油可能是一种有效的抗菌冲洗液。
