Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Prep Biochem Biotechnol. 2021;51(10):971-978. doi: 10.1080/10826068.2021.1881907. Epub 2021 Feb 13.
(SKJ), which is a member of , has various proven effects such as antispasmodic and anti-inflammatory, anti-diabetic, antioxidant, and antifungal properties. However, the use of essential oil of plants is limited due to their inherent instability in the environment. Encapsulation with nanoparticles in the nanogel forms is one of their stabilization methods. The aim of this study was to synthesize nano-gel based on chitosan (CS) and extracts of SKJ essential oil, and to evaluate the antibacterial and anticancer activities. SKJ essential oil was extracted using water distillation method. Then, it was loaded on CS particles using two-step process as following: droplets formation and freezing. The Dynamic Light Scattering (DLS), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), and Zeta potential determination were used to evaluate the physical and chemical properties of CS-SKJ nanogel, which its result was acceptable. After confirmation of the loaded essential oil rate and releasing amount, the antibacterial effects were evaluated on five Gram-positive bacteria and five Gram-negative bacteria using microbroth dilution method. The encapsulation efficiency, size, polydispersity index, and zeta potential of nanoparticles were characterized were 30.74%, 571.00 nm, 0.451 and -67.2 mV, respectively. The results were significant not only on Gram-positive bacteria, but also on Gram-negative bacteria. The MIC range was between 7.8 and 500 µg/ml. The CS-SKJ nanogel has acceptable anticancer activities on KB and A549 tumor cell lines. the IC50 range was between 5.6 and 6.71 µg/ml. The results indicate that both CS particles and SKJ alone, and CS-SKJ nanogel could be considered as the outlook to produce new antimicrobial and anticancer drugs.
(SKJ) 是 的成员，具有多种已证实的功效，如抗痉挛、抗炎、抗糖尿病、抗氧化和抗真菌特性。然而，由于其在环境中的固有不稳定性，植物精油的使用受到限制。将其封装在纳米凝胶形式的纳米颗粒中是其稳定化方法之一。本研究的目的是合成基于壳聚糖 (CS) 和 SKJ 精油提取物的纳米凝胶，并评估其抗菌和抗癌活性。SKJ 精油采用水蒸馏法提取。然后，通过两步法将其负载到 CS 颗粒上，即液滴形成和冷冻。动态光散射 (DLS)、傅里叶变换红外光谱 (FTIR)、扫描电子显微镜 (SEM) 和 Zeta 电位测定用于评估 CS-SKJ 纳米凝胶的物理和化学性质，结果是可接受的。在确认负载精油的比率和释放量后，采用微量稀释法评估了 CS-SKJ 纳米凝胶对五种革兰氏阳性菌和五种革兰氏阴性菌的抗菌作用。纳米粒子的包封效率、粒径、多分散指数和 Zeta 电位分别为 30.74%、571.00nm、0.451 和-67.2mV。结果不仅对革兰氏阳性菌而且对革兰氏阴性菌都有显著影响。MIC 范围在 7.8 至 500μg/ml 之间。CS-SKJ 纳米凝胶对 KB 和 A549 肿瘤细胞系具有可接受的抗癌活性。IC50 范围在 5.6 至 6.71μg/ml 之间。结果表明，CS 颗粒和 SKJ 单独以及 CS-SKJ 纳米凝胶都可以被认为是生产新的抗菌和抗癌药物的前景。
