Sharifian Mohammad Reza, Shokouhinejad Noushin, Aligholi Marzieh, Emaneini Mohammad, Alizadeh Javad
Department of Endodontics, Faculty of Dentistry/Dental Research Center, Tehran University of Medical Sciences, and member of Iranian Center for Endodontic Research, Tehran, Iran.
Iran Endod J. 2009 Spring;4(2):45-8. Epub 2009 Apr 17.
Various methods commonly used for cleaning and shaping root canals have not been successful in completely eradicating bacteria due to anatomic complexity and root canals irregularities. Disinfecting the canals with intracanal irrigants in addition to proper cleaning and shaping can produce a successful outcome. Antimicrobials with sustained antibacterial activity would be desirable for irrigation. The purpose of this study was to compare the antimicrobial substantivity of Carvacrol and 5.25% NaOCl in infected bovine root dentin.
One hundred and twenty dentin tubes prepared from bovine incisors were infected in vitro for 14 days with Enterococcus (E) faecalis. The specimens were divided into four groups including 1) Carvacrol, 2) NaOCl, 3) infected dentin tubes (positive control); and 4) sterile dentin tubes (negative control). Dentin chips were collected at five intervals (days 0, 1, 3, 7 and 28) using round burs with sequentially increasing diameters (which includes five layers of dentin) into Brain Heart Infusion (BHI) broth. In order to compare the pre- and post-irrigation antimicrobial activity of the irrigants the colony-forming units (CFU) were counted and classified as 'CFU-before' and 'CFU-after'. After culturing, the number of CFU with the various experimental time and dentinal layers was recounted. Two-way ANOVA test was used to analyze the effects of time and materials. One-way ANOVA and supplemental Tukey HSD test were used for pair comparison.
CFU was significantly reduced in NaOCl group when compared to all other experimental groups (P<0.05).
The substantivity of NaOCl was significantly greater than Carvacrol. Further studies are required to investigate and approve Carvacrol as a final irrigant.
由于解剖结构复杂和根管不规则，常用于清洁和塑形根管的各种方法未能成功完全根除细菌。除了适当的清洁和塑形外，用根管内冲洗剂对根管进行消毒可产生成功的结果。具有持续抗菌活性的抗菌剂将是理想的冲洗剂。本研究的目的是比较香芹酚和5.25%次氯酸钠在感染牛牙根本质中的抗菌持久性。
用牛切牙制备120根管状牙本质，在体外用人粪肠球菌感染14天。标本分为四组，包括1）香芹酚组，2）次氯酸钠组，3）感染牙本质管组（阳性对照组）；4）无菌牙本质管组（阴性对照组）。使用直径依次增大的圆钻（包括五层牙本质）在五个时间间隔（第0、1、3、7和28天）收集牙本质碎屑，放入脑心浸液（BHI）肉汤中。为了比较冲洗剂冲洗前后的抗菌活性，对菌落形成单位（CFU）进行计数，并分为“冲洗前CFU”和“冲洗后CFU”。培养后，重新计算不同实验时间和牙本质层的CFU数量。采用双向方差分析检验分析时间和材料的影响。采用单向方差分析和补充的Tukey HSD检验进行配对比较。
与所有其他实验组相比，次氯酸钠组的CFU显著降低（P<0.05）。
次氯酸钠的持久性显著大于香芹酚。需要进一步研究以研究和批准香芹酚作为最终冲洗剂。
