Department of Urology, Qilu Hospital, Shandong University, 107# Wenhua Xi Road, Jinan, 250012 PR China.
World J Surg Oncol. 2011 Dec 2;9:158. doi: 10.1186/1477-7819-9-158.
Tumor angiogenesis is important in the progression of malignancies, and heparanase plays an important role in sustaining the pathology of clear cell renal cell cancer (ccRCC). The study was carried out to investigate the correlations between microvessel density (MVD) and heparanase expression containing prognostic significances in the patients with ccRCC.
Specimens from 128 patients with ccRCC were investigated by immunohistochemistry for MVD. RT-PCR and immunohistochemistry were used to detect heparanase expression. Correlations between MVD, heparanase expression, and various clinico-pathological factors were studied. The prognostic significances of MVD and heparanase expression were also analysed.
We discovered a statistically significant prevalence of higher MVD in ccRCC compared with adjacent normal renal tissues. MVD was positively correlated with TNM stage and distant metastasis in ccRCC patients, and was also correlated with the expression level of heparanase.Heparanase is over-expressed and correlated with TNM stage, histologic grade, distant metastasis and lymphatic metastasis in ccRCC. High MVD and heparanase over-expression inversely correlate with the survival of ccRCC patients.
Heparanase contributes to angiogenesis of ccRCC and over-expression of heparanase is an independent predictors of prognosis for ccRCC. MVD is correlated with tumor development and metastasis in ccRCC.
肿瘤血管生成在恶性肿瘤的进展中很重要，肝素酶在维持透明细胞肾细胞癌（ccRCC）的病理学方面起着重要作用。本研究旨在探讨 ccRCC 患者中微血管密度（MVD）与具有预后意义的肝素酶表达之间的相关性。
通过免疫组织化学法检测 128 例 ccRCC 患者的 MVD。使用 RT-PCR 和免疫组织化学法检测肝素酶的表达。研究 MVD、肝素酶表达与各种临床病理因素之间的相关性。还分析了 MVD 和肝素酶表达的预后意义。
与相邻正常肾组织相比，我们发现 ccRCC 中 MVD 的发生率明显更高。在 ccRCC 患者中，MVD 与 TNM 分期和远处转移呈正相关，并且与肝素酶的表达水平相关。肝素酶在 ccRCC 中过度表达，与 TNM 分期、组织学分级、远处转移和淋巴转移相关。高 MVD 和肝素酶过度表达与 ccRCC 患者的生存呈负相关。
肝素酶有助于 ccRCC 的血管生成，肝素酶的过度表达是 ccRCC 预后的独立预测因子。MVD 与 ccRCC 中的肿瘤发展和转移相关。
