Laboratorio de Células y Cultivo Tejidos, Centro de Bioplantas, Universidad de Ciego de Ávila, Ciego de Ávila, Cuba.
Plant Cell Rep. 2012 Apr;31(4):757-69. doi: 10.1007/s00299-011-1195-7. Epub 2011 Dec 2.
Many plant species grown under in vitro controlled conditions can be used as models for the study of physiological processes. Adult pineapple can display CAM physiology while in vitro it functions as a C3 plant. Ex vitro Ananas comosus has plastic morphology and physiology, both easy to modify from C3 to CAM by changing the environmental conditions. The yield of survival for a rentable propagation protocol of pineapple is closely related with the C3/CAM shift and the associated physiological characteristics. In the present work, ex vitro pineapple plants were divided in two sets and subjected to C3 and CAM-inducing environmental conditions, determined by light intensity and relative humidity, respectively, 40 μmol m(-2) s(-1)/85% and 260 μmol m(-2) s(-1)/50%. The results demonstrated that the stress imposed by the environmental conditions switched pineapple plants from C3 to CAM behavior. Comparing to CAM induced, C3-induced pineapple plants showed substandard growth parameters and morphological leaf characteristics but a better rooting process and a higher ABA production, a phenotype closer to adult plants, which are expected to produce fruits in a normal production cycle. We conclude that the upholding of these characteristics is conditioned by low light intensity plus high relative humidity, especially during the first 8 weeks of ex vitro growth. It is expected that the better understanding of pineapple acclimatization will contribute to the design of a protocol to apply as a rentable tool in the pineapple agronomic industry.
许多在体外控制条件下生长的植物物种可以被用作生理过程研究的模型。成年菠萝在体外表现为 CAM 生理特性，而在体内则表现为 C3 植物。离体的菠萝具有可塑性的形态和生理特性，通过改变环境条件，很容易从 C3 转变为 CAM。菠萝可租赁繁殖方案的成活率与 C3/CAM 转变和相关生理特性密切相关。在本工作中，离体的菠萝植株被分为两组，分别在光强和相对湿度分别为 40 μmol m(-2) s(-1)/85%和 260 μmol m(-2) s(-1)/50%的环境条件下进行 C3 和 CAM 诱导。结果表明，环境条件施加的胁迫使菠萝植株从 C3 转变为 CAM 行为。与 CAM 诱导相比，C3 诱导的菠萝植株表现出较差的生长参数和形态叶片特征，但生根过程较好，ABA 产量较高，更接近成年植株的表型，预计在正常的生产周期中会产生果实。我们得出结论，这些特性的维持受到低光强加高光相对湿度的影响，特别是在离体生长的前 8 周。我们期望对菠萝适应的更好理解将有助于设计一种协议，作为菠萝农业产业中一种可行的工具应用。
