Wai Ching M, VanBuren Robert, Zhang Jisen, Huang Lixian, Miao Wenjing, Edger Patrick P, Yim Won C, Priest Henry D, Meyers Blake C, Mockler Todd, Smith J Andrew C, Cushman John C, Ming Ray
FAFU and UIUC Joint Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Key Laboratory of Genetics, Breeding and Multiple Utilization of Corps, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Plant J. 2017 Oct;92(1):19-30. doi: 10.1111/tpj.13630. Epub 2017 Aug 21.
The altered carbon assimilation pathway of crassulacean acid metabolism (CAM) photosynthesis results in an up to 80% higher water-use efficiency than C photosynthesis in plants making it a potentially useful pathway for engineering crop plants with improved drought tolerance. Here we surveyed detailed temporal (diel time course) and spatial (across a leaf gradient) gene and microRNA (miRNA) expression patterns in the obligate CAM plant pineapple [Ananas comosus (L.) Merr.]. The high-resolution transcriptome atlas allowed us to distinguish between CAM-related and non-CAM gene copies. A differential gene co-expression network across green and white leaf diel datasets identified genes with circadian oscillation, CAM-related functions, and source-sink relations. Gene co-expression clusters containing CAM pathway genes are enriched with clock-associated cis-elements, suggesting circadian regulation of CAM. About 20% of pineapple microRNAs have diel expression patterns, with several that target key CAM-related genes. Expression and physiology data provide a model for CAM-specific carbohydrate flux and long-distance hexose transport. Together these resources provide a list of candidate genes for targeted engineering of CAM into C photosynthesis crop species.
景天酸代谢（CAM）光合作用中改变的碳同化途径，使得植物的水分利用效率比C3光合作用高出80%，这使其成为一种潜在有用的途径，可用于培育具有更强耐旱性的作物。在此，我们详细调查了专性CAM植物菠萝[Ananas comosus (L.) Merr.]的时间（昼夜时间进程）和空间（叶片梯度）基因及微小RNA（miRNA）表达模式。高分辨率转录组图谱使我们能够区分与CAM相关和非CAM的基因拷贝。通过对绿色和白色叶片昼夜数据集的差异基因共表达网络分析，鉴定出具有昼夜振荡、CAM相关功能以及源库关系的基因。包含CAM途径基因的基因共表达簇富含与生物钟相关的顺式元件，表明CAM受昼夜节律调控。约20%的菠萝miRNA具有昼夜表达模式，其中一些靶向关键的CAM相关基因。表达和生理数据为CAM特异性碳水化合物通量和长距离己糖运输提供了一个模型。这些资源共同提供了一份候选基因清单，用于将CAM定向工程导入C3光合作用作物物种。
