Plant Cell Rep. 2013 Nov;32(11):1807-18. doi: 10.1007/s00299-013-1493-3.
Proteomic and transcriptomic profiles of key enzymes were monitored in pineapple plants propagated under C3 and CAM-inducing metabolisms to obtain insight into the CAM-facultative metabolism and the relationship of CAM plants with oxidative stress.
Pineapple is one of the most important tropical crops worldwide. The use of temporary immersion bioreactors for the first stages of pineapple propagation enables precise control of plant growth, increases the rate of plant multiplication, decreases space, energy and labor requirements for pineapple plants in commercial micropropagation. Once the plantlets are ready to be taken from the reactors, they are carefully acclimatized to natural environmental conditions, and a facultative C3/CAM metabolism in the first 2 months of growth is the characteristic of pineapple plants, depending on environmental conditions. We subjected two sets of micropropagated pineapple plants to C3 and CAM-inducing environmental conditions, determined by light intensity/relative humidity (respectively 40 μmol m−2 s−1/85 % and 260 μmol m−2 s−1/50 %). Leaves of pineapple plants grown under CAM-inducing conditions showed higher leaf thickness and more developed cuticles and hypodermic tissue. Proteomic profiles of several proteins, isoenzyme patterns and transcriptomic profiles were also measured. Five major spots were isolated and identified, two of them for the first time in Ananas comosus (OEE 1; OEE 2) and the other three corresponding to small fragments of the large subunit of Rubisco (LSU). PEPC and PEPCK were also detected by immunobloting of 2DE at the end of both ex vitro treatments (C3/CAM) during the dark period. Isoenzymes of SOD and CAT were identified by electrophoresis and the transcript levels of OEE 1 and CAT were associated with CAM metabolism in pineapple plants.
在菠萝植物中监测关键酶的蛋白质组学和转录组学谱，以深入了解 CAM 兼性代谢以及 CAM 植物与氧化应激的关系。
菠萝是全球最重要的热带作物之一。使用临时浸入生物反应器进行菠萝繁殖的早期阶段，可以精确控制植物生长，提高植物繁殖速度，减少商业微繁殖中菠萝植物对空间、能源和劳动力的需求。一旦植物准备好从反应器中取出，它们就会被小心地适应自然环境条件，在生长的前 2 个月，兼性 C3/CAM 代谢是菠萝植物的特征，这取决于环境条件。我们将两组微繁殖的菠萝植物置于 C3 和 CAM 诱导的环境条件下，由光照强度/相对湿度（分别为 40 μmol m−2 s−1/85%和 260 μmol m−2 s−1/50%）决定。在 CAM 诱导条件下生长的菠萝叶片表现出更高的叶片厚度和更发达的角质层和下皮层。还测量了几种蛋白质、同工酶模式和转录组学谱的蛋白质组学谱。分离并鉴定了五个主要斑点，其中两个是在菠萝中首次发现的（OEE 1；OEE 2），另外三个对应于 Rubisco 大亚基的小片段（LSU）。在暗期结束时，通过 2DE 的免疫印迹还检测到了 PEPC 和 PEPCK。通过电泳鉴定了 SOD 和 CAT 的同工酶，并且 OEE 1 和 CAT 的转录水平与菠萝植物中的 CAM 代谢相关。
