南非长颈鹿和南非大羚羊皮肤损伤中乳头瘤病毒的检测与特征分析

Detection and characterisation of papillomavirus in skin lesions of giraffe and sable antelope in South Africa.

作者信息

van Dyk E, Bosman A M, van Wilpe E, Williams J H, Bengis R G, van Heerden J, Venter E H

机构信息

Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa.

出版信息

J S Afr Vet Assoc. 2011 Jun;82(2):80-5. doi: 10.4102/jsava.v82i2.39.

DOI:10.4102/jsava.v82i2.39

PMID:22135920

Abstract

Papillomavirus was detected electron microscopically in cutaneous fibropapillomas of a giraffe (Giraffa camelopardalis) and a sable antelope (Hippotragus niger). The virus particles measured 45 nm in diameter. Histopathologically, the lesions showed histopathological features similar to those of equine sarcoid as well as positive immunoperoxidase-staining of tissue sections for papillomavirus antigen. Polymerase chain reaction (PCR) detected bovine papillomavirus (BPV) DNA. Bovine papillomavirus-1 was characterised by real-time PCR in the sable and giraffe, and cloning and sequencing of the PCR product revealed a similarity to BPV-1. As in the 1st giraffe, the lesions from a 2nd giraffe revealed locally malignant pleomorphism, possibly indicating the lesional end-point of papilloma infection. Neither virus particles nor positively staining papillomavirus antigen could be demonstrated in the 2nd giraffe but papillomavirus DNA was detected by real-time PCR which corresponded with BPV-1 and BPV-2.

摘要

通过电子显微镜在一头长颈鹿（长颈鹿属）和一头貂羚（黑马羚属）的皮肤纤维乳头瘤中检测到乳头瘤病毒。病毒颗粒直径为45纳米。组织病理学上，病变显示出与马肉瘤相似的组织病理学特征，并且组织切片对乳头瘤病毒抗原的免疫过氧化物酶染色呈阳性。聚合酶链反应（PCR）检测到牛乳头瘤病毒（BPV）DNA。在貂羚和长颈鹿中通过实时PCR对牛乳头瘤病毒1型进行了鉴定，PCR产物的克隆和测序显示与BPV-1相似。与第一头长颈鹿一样，第二头长颈鹿的病变显示出局部恶性多形性，这可能表明乳头瘤感染的病变终点。在第二头长颈鹿中既未检测到病毒颗粒，也未检测到呈阳性染色的乳头瘤病毒抗原，但通过实时PCR检测到了乳头瘤病毒DNA，其与BPV-1和BPV-2相符。