中国熊蜂属（Bombus spp.）共生的微孢子虫多样性。
Diversity of Nosema associated with bumblebees (Bombus spp.) from China.
机构信息
Key Laboratory of Pollinating Insect Biology of the Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Science, Xiangshan, Beijing 100093, China.
出版信息
Int J Parasitol. 2012 Jan;42(1):49-61. doi: 10.1016/j.ijpara.2011.10.005. Epub 2011 Nov 22.
Bumblebees (Bombus spp.) are important pollinators of many economically important crops and microsporidia are among the most important infections of these hosts. Using molecular markers, we screened a large sample (n=1,009 bees) of workers of 27 different Bombus spp. from China (Sichuan, Qinghai, Inner Mongolia, and Gansu provinces). The results showed that 62 individuals representing 12 Bombus spp. were infected by microsporidia with an overall prevalence of 6.1%. Based on the haplotypes (ssrRNA sequences), we confirmed the presence of Nosema bombi, Nosema ceranae and (likely) Nosema thomsoni. In addition, four new putatively novel taxa were identified by phylogenetic reconstruction: Nosema A, Nosema B-complex, Nosema C-complex and Nosema D-complex. In many cases, hosts were infected by more than one Nosema taxon. Possible caveats of sequence analyses are discussed.
熊蜂（Bombus spp.）是许多重要经济作物的重要传粉媒介，而微孢子虫则是这些宿主最重要的感染源之一。本研究利用分子标记，对来自中国（四川、青海、内蒙古和甘肃等省）的 27 种不同熊蜂属（Bombus spp.）的 1009 只工蜂进行了大规模样本筛查。结果显示，62 只代表 12 种熊蜂的个体受到微孢子虫感染，总体感染率为 6.1%。基于单核苷酸多态性（ssrRNA 序列），我们确认存在布氏微孢子虫（Nosema bombi）、中华蜜蜂微孢子虫（Nosema ceranae）和（可能）感染了 Thomson 蜜蜂微孢子虫（Nosema thomsoni）。此外，通过系统发育重建还鉴定出了 4 种新的推定新型分类单元：Nosema A、Nosema B 复合体、Nosema C 复合体和 Nosema D 复合体。在许多情况下，宿主被不止一种微孢子虫感染。本研究还讨论了序列分析可能存在的局限性。
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