Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Key Laboratory of Marine Bio-resources Sustainable Utilization, CAS, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.
Int J Syst Evol Microbiol. 2012 Oct;62(Pt 10):2395-2399. doi: 10.1099/ijs.0.037028-0. Epub 2011 Nov 18.
A Gram-positive, non-motile, endophytic actinomycete, designated strain YIM 63638(T), was isolated from the surface-sterilized roots of Artemisia annua L. The isolate grew optimally with 1-3 % (w/v) NaCl, at pH 6.0-7.0 and at 20-37 °C. Comparative analysis of the 16S rRNA gene sequence showed that the isolate belonged to the genus Pseudonocardia and showed highest sequence similarity with Pseudonocardia oroxyli D10(T) (98.9 %), Pseudonocardia ailaonensis YIM 45505(T) (98.3 %) and Pseudonocardia halophobica IMSNU 21327(T) (98.0 %). Phylogenetic distance from other type strains of species with validly published names within the genus Pseudonocardia was greater than 2.3 %. Strain YIM 63638(T) had a genomic DNA G+C content of 72.1 mol% and MK-8(H(4)) as the predominant respiratory quinone. The major fatty acids were iso-C(16 : 0) (36.32 %) and 10-methyl-C(16 : 0) (19.78 %). On the basis of phenotypic properties and phylogenetic distinctiveness, strain YIM 63638(T) represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia xishanensis sp. nov. is proposed. The type strain is YIM 63638(T) ( = JCM 17906(T) = KCTC 29005(T)).
一株革兰氏阳性、非运动、内生放线菌，命名为 YIM 63638(T)，从青蒿的表面消毒根部分离得到。该分离物在 1-3%（w/v）NaCl、pH6.0-7.0 和 20-37°C 的条件下生长最佳。16S rRNA 基因序列的比较分析表明，该分离物属于假诺卡氏菌属，与假诺卡氏菌奥罗利 D10(T)（98.9%）、假诺卡氏菌 YIM 45505(T)（98.3%）和假诺卡氏菌嗜盐菌 IMSNU 21327(T)（98.0%）的序列相似性最高。与该属内已发表有效名称的其他模式菌株的系统发育距离大于 2.3%。菌株 YIM 63638(T)的基因组 DNA G+C 含量为 72.1mol%，主要呼吸醌为 MK-8(H(4))。主要脂肪酸为 iso-C(16 : 0)（36.32%）和 10-甲基-C(16 : 0)（19.78%）。基于表型特征和系统发育差异，菌株 YIM 63638(T)代表假诺卡氏菌属的一个新种，命名为假诺卡氏菌西山新种。其模式菌株为 YIM 63638(T)（ = JCM 17906(T) = KCTC 29005(T)）。
