德尔塔病毒RNA序列的异质性和进化速率。
Heterogeneity and evolution rates of delta virus RNA sequences.
作者信息
Imazeki F, Omata M, Ohto M
机构信息
First Department of Medicine, Chiba University School of Medicine, Japan.
出版信息
J Virol. 1990 Nov;64(11):5594-9. doi: 10.1128/JVI.64.11.5594-5599.1990.
To investigate the geographical divergence of delta virus RNA sequences, 868 nucleotides (nt), including the delta antigen-coding region, were determined in isolates from two Japanese patients, M and S, by polymerase chain reaction and direct sequencing and compared with three previously reported nucleotide sequences. The sequence obtained for hepatitis delta virus RNA from patient M was approximately 92% identical to sequences previously obtained for two other strains of hepatitis delta virus, whereas the sequence of hepatitis delta virus RNA obtained from patient S was approximately 81% identical to the previously sequenced strains. This suggests that delta agent in Japan has a heterogeneous origin and the delta virus RNA sequence from Japanese patient S is the most divergent delta virus isolate yet analyzed. To study the evolution rate of delta virus RNA, viral isolates obtained 3 and 4 years apart from each of two patients were also sequenced. It was estimated that the substitution rate of viral RNA was 0.57 x 10(-3) nt per site per year in patient M and 0.64 x 10(-3) nt per site per year in patient S for the delta antigen gene.
为研究丁型肝炎病毒RNA序列的地理差异，通过聚合酶链反应和直接测序法，测定了两名日本患者（M和S）分离株中包括丁型肝炎抗原编码区在内的868个核苷酸（nt），并与之前报道的三个核苷酸序列进行比较。患者M的丁型肝炎病毒RNA序列与之前获得的另外两株丁型肝炎病毒序列的相似度约为92%，而患者S的丁型肝炎病毒RNA序列与之前测序的毒株的相似度约为81%。这表明日本的丁型肝炎病毒具有异源起源，且来自日本患者S的丁型肝炎病毒RNA序列是目前分析过的最具差异的丁型肝炎病毒分离株。为研究丁型肝炎病毒RNA的进化速率，还对分别来自两名患者、间隔3年和4年获得的病毒分离株进行了测序。据估计，患者M的丁型肝炎抗原基因的病毒RNA替换率为每年每位点0.57×10⁻³ nt，患者S为每年每位点0.64×10⁻³ nt。
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