Chen Hai-e, Xiao Dun-zhen
Research Institute of Family Planning, Tongjin Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Zhonghua Nan Ke Xue. 2011 Nov;17(11):1002-6.
To investigate the effect of diabetic autonomic neuropathy on the seminal vesicle and search for the theoretical evidence for the prevention and treatment of diabetic infertility by observing changes in the contents of the nerve growth factor (NGF) and muscarinic M3 receptor in the seminal vesicle of diabetic rats.
Diabetic models were established in 10 of the 15 male adult SD rats by intraperitoneal injection of streptozotocin (STZ), and the other 5 were included in a normal control group. Eight weeks after modeling, seminal vesicles were collected from the rats for HE and immunohistochemical staining.
Compared with the normal controls, the diabetic models showed a decreased number of smooth muscle cells, thinner cytoplasm of glandular epithelial cells and disordered structure in the seminal vesicle. The intensity of NGF-positive staining was significantly enhanced, but that of M3 markedly reduced in the diabetic group. There were statistically significant differences in the mean integrated optical density (IA) of muscarinic M3 receptors and NGF between the control and diabetic groups (0.0187 +/- 0.0024 vs 0.0100 +/- 0.0015 and 0.0209 +/- 0.0085 vs 0.0412 +/- 0.0117, P<0.01).
The changes in the expressions of NGF and M3 receptors in the seminal vesicle of diabetic rats suggest that diabetes mellitus may induce autonomic neuropathy of the seminal vesicle.
通过观察糖尿病大鼠精囊神经生长因子（NGF）和毒蕈碱M3受体含量的变化，探讨糖尿病自主神经病变对精囊的影响，为糖尿病性不育症的防治寻找理论依据。
15只成年雄性SD大鼠中，10只通过腹腔注射链脲佐菌素（STZ）建立糖尿病模型，另外5只作为正常对照组。造模8周后，采集大鼠精囊进行HE染色和免疫组化染色。
与正常对照组相比，糖尿病模型组精囊平滑肌细胞数量减少，腺上皮细胞胞质变薄，结构紊乱。糖尿病组NGF阳性染色强度显著增强，而M3阳性染色强度明显降低。对照组和糖尿病组毒蕈碱M3受体和NGF的平均积分光密度（IA）差异有统计学意义（0.0187±0.0024对0.0100±0.0015，0.0209±0.0085对0.0412±0.0117，P<0.01）。
糖尿病大鼠精囊中NGF和M3受体表达的变化提示糖尿病可能诱发精囊自主神经病变。
