Women's Reproductive Health Research Center, Department of Pediatrics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Biol Trace Elem Res. 2012 Jun;147(1-3):1-7. doi: 10.1007/s12011-011-9270-z. Epub 2011 Dec 6.
Bronchopulmonary dysplasia (BPD), also known as chronic lung disease, is one of the most challenging complications in premature newborn infants. Selenium plays a role in antioxidant system by protecting cell membranes and neutralizing the deleterious effects of free radicals. The aim of this study was to determine the relationship between selenium concentration and incidence of bronchopulmonary dysplasia using a validated analytical method. Umbilical cord blood and blood samples 30 days after the birth were collected from 38 preterm newborn infants with gestation age of 32 weeks or less, and the separated serums were kept at -70°C until analysis time. Selenium concentration of serum was determined using an atomic absorption spectrophotometer. The method was validated on the basis of standard validation techniques. The analytical method was linear in the range of 1 to 500 μg/L with the limit of detection of 0.4 μg/L. Samples were collected from 38 infants whose gestation age was 32 weeks or less. The blood samples were collected from the umbilical cord blood at birth in 19 cases. In 25 cases, blood samples were collected 1 month after birth. Of the 15 patients diagnosed with BPD, 10 were boys (p = 0.02). The mean serum selenium concentration was not different at birth between patients with and without BPD, but it was significantly lower at 30 days after birth in patients with BPD (38.5 ± 14.1vs. 45.4 ± 18.7 μg/L, p = 0.02). Preterm newborn infants with BPD had lower serum selenium concentrations 1 month after birth.
支气管肺发育不良（BPD），也称为慢性肺部疾病，是早产儿最具挑战性的并发症之一。硒通过保护细胞膜和中和自由基的有害作用，在抗氧化系统中发挥作用。本研究旨在使用经过验证的分析方法确定硒浓度与支气管肺发育不良发生率之间的关系。从胎龄为 32 周或以下的 38 例早产儿中采集脐带血和出生后 30 天的血液样本，并将分离的血清在-70°C 下保存，直至分析时间。使用原子吸收分光光度计测定血清中的硒浓度。该方法基于标准验证技术进行了验证。分析方法在 1 至 500μg/L 范围内呈线性，检测限为 0.4μg/L。从胎龄为 32 周或以下的 38 名婴儿中采集样本。在 19 例中，在出生时从脐血中采集血液样本。在 25 例中，在出生后 1 个月采集血液样本。在 15 名被诊断为 BPD 的患者中，有 10 名是男孩（p=0.02）。在患有 BPD 的患者和没有 BPD 的患者之间，出生时的血清硒浓度没有差异，但在患有 BPD 的患者中，出生后 30 天的血清硒浓度明显较低（38.5±14.1vs.45.4±18.7μg/L，p=0.02）。患有 BPD 的早产儿在出生后 1 个月时血清硒浓度较低。
