Ding Shinghua
Department of Biological Engineering, Dalton Cardiovascular Research Center, University of Missouri-Columbia, Columbia, MO, USA.
Methods Mol Biol. 2012;814:545-54. doi: 10.1007/978-1-61779-452-0_36.
Astrocytes are the predominant nonneuronal cell type in the central nervous system. Although they are electrically nonexcitable, they have been found to play an active role in modulation of neuronal function and plasticity through Ca(2+) excitability. Thus, Ca(2+) signaling in astrocytes serves as a mediator of bidirectional interactions between neurons and astrocytes. Although astrocytic Ca(2+) signaling has been extensively studied in cultured cells, the recent development of two-photon laser scanning fluorescent microscopy and astrocyte-specific dye labeling make it possible to study astrocytic Ca(2+) signaling in live animals. Here we describe a detailed protocol for in vivo Ca(2+) imaging of astrocytes in mice.
星形胶质细胞是中枢神经系统中主要的非神经元细胞类型。尽管它们在电方面不可兴奋，但已发现它们通过Ca(2+) 兴奋性在调节神经元功能和可塑性方面发挥积极作用。因此，星形胶质细胞中的Ca(2+) 信号传导充当神经元和星形胶质细胞之间双向相互作用的介质。尽管在培养细胞中对星形胶质细胞Ca(2+) 信号传导进行了广泛研究，但双光子激光扫描荧光显微镜和星形胶质细胞特异性染料标记的最新进展使得在活体动物中研究星形胶质细胞Ca(2+) 信号传导成为可能。在这里，我们描述了一种用于小鼠星形胶质细胞体内Ca(2+) 成像的详细方案。
