Zahalak G I, McConnaughey W B, Elson E L
Department of Mechanical Engineering, Washington University, St. Louis, MO 63130.
J Biomech Eng. 1990 Aug;112(3):283-94. doi: 10.1115/1.2891186.
In this paper we review the cell-poking technique as an approach for investigating the mechanical properties of living cells. We first summarize the rationale for the technique and the mainly qualitative results obtained so far. Then we provide a technical description of the instrument as it is configured at present. This is followed by a discussion of the current status of analytical results available for interpreting cell-poking measurements. In the final section we apply these results to an analysis of unmodulated and modulated lymphocytes and neutrophils, and conclude that the mechanical response of these leukocytes to indentation is not consistent with simple models developed by previous investigators on the basis of micropipette-aspiration experiments.
在本文中，我们回顾了细胞戳压技术，该技术作为一种研究活细胞力学特性的方法。我们首先总结了该技术的基本原理以及迄今为止所获得的主要定性结果。然后，我们对目前配置的仪器进行技术描述。接下来讨论可用于解释细胞戳压测量结果的分析结果的现状。在最后一部分，我们将这些结果应用于对未调制和调制淋巴细胞及中性粒细胞的分析，并得出结论：这些白细胞对压痕的力学响应与先前研究人员基于微量吸管抽吸实验开发的简单模型不一致。
