United Blood Services of the Rio Grande Valley, 1400 S.6th St., McAllen, TX 78501, USA.
Transfusion. 2011 Dec;51(12 Pt 2):2758-60. doi: 10.1111/j.1537-2995.2011.03444.x.
A multisite blood center experienced unacceptable post-leukoreduction filtration white blood cell (WBC) counts at a few centers. Since prefiltration storage time and temperature were suspect, whole blood (WB) units were stored in transport shippers for at least 2 hours, cooling toward 1-6 ° C, before filtration. This study compared the effect of storage times in transport shippers on the residual WBC counts of leukoreduced units.
Collection and filtration of WB units were accomplished with the use of the Fenwal Express System with Integral Sepacell RZ-2000 WB Leukocyte Reduction Filter. Units were collected and placed in transport shippers containing ice. Leukoreduction filtration was performed at designated intervals post-collection. Acceptable leukoreduction was defined as < 5 × 10(6) residual WBC.
Fifty donor units were selected randomly over 3 months. Units were held in transport shippers, and WBC reduction was performed at designated post-collection intervals. Storage times ranged from 28 to 458 minutes. All residual WBC counts were acceptable.
Storage time of WB units in transport shippers did not play a role in the efficacy of the leukoreduction. This study demonstrated the 2-hour storage time before leukoreduction filtration could be eliminated resulting in time savings and increased efficacy in the component production laboratory.
一个多站点血液中心在几个中心经历了不可接受的白细胞过滤后（WBC）计数。由于预过滤储存时间和温度存在问题，全血（WB）单位在过滤前至少在运输容器中储存 2 小时，冷却至 1-6°C。本研究比较了在运输容器中储存时间对白细胞减少单位残留 WBC 计数的影响。
使用 Fenwal Express 系统和 Integral Sepacell RZ-2000 WB 白细胞减少过滤器收集和过滤 WB 单位。单位收集后放置在装有冰的运输容器中。收集后指定时间进行白细胞减少过滤。可接受的白细胞减少定义为 < 5 × 10(6) 残留 WBC。
在 3 个月内随机选择了 50 个供体单位。单位放置在运输容器中，并在指定的收集后间隔进行 WBC 减少处理。储存时间从 28 分钟到 458 分钟不等。所有残留的 WBC 计数都是可接受的。
WB 单位在运输容器中的储存时间不会影响白细胞减少的效果。本研究表明，在进行白细胞减少过滤之前，可以消除 2 小时的储存时间，从而节省时间并提高成分生产实验室的效率。
