Grundy S M, Mok H Y, Zech L, Steinberg D, Berman M
J Clin Invest. 1979 Jun;63(6):1274-83. doi: 10.1172/JCI109422.
Measurements of transport of triglycerides (TG) in very low density lipoproteins (VLDL) were carried out in 59 patients by injection of radioactive glycerol, determinations of specific activities of VLDL-TG for 48 h thereafter, and treatment of the data by multicompartmental analysis. The patients were divided into three groups: normal weight (89-120% ideal weight), mildly obese (120-135% ideal weight), and markedly obese (135% ideal weight). They had varying levels of VLDL-TG ranging from normal to markedly elevated. In many subjects, there was a positive correlation between concentrations and transport of VLDL indicating that overproduction of VLDL-TG contributed to hypertriglyceridemia. In others, and particularly in several markedly obese subjects, transport rates were greatly increased without significant hypertriglyceridemia, suggesting that they had enhanced capacity to clear TG. In all groups, however, there were patients whose degree of hypertriglyceridemia seemed out of proportion to their transport rates. This finding and the fact that many patients have increased secretion of VLDL-TG without elevated plasma TG suggests that both overproduction of VLDL-TG and insufficient enhancement of clearance contributed to the development of hypertriglyceridemia.The data showed a poor correlation between transport rates determined by our multicompartment analysis and single-exponential analysis used previously by other investigators (r = 0.46); this comparison was not improved by segregating patients according to their degree of obesity. Although two conversion pathways (fast and slow synthetic paths) were required to fit the data, there was no correlation between transport rates and the ratio of the two pathways. Also, despite the known pathway of conversion of VLDL to low density lipoprotein, no correlation was found between VLDL-TG transport rates and estimated low density lipoprotein-cholesterol concentrations.
通过注射放射性甘油对59例患者进行极低密度脂蛋白（VLDL）中甘油三酯（TG）转运的测量，随后测定48小时内VLDL-TG的比活性，并通过多室分析处理数据。患者分为三组：正常体重（理想体重的89 - 120%）、轻度肥胖（理想体重的120 - 135%）和显著肥胖（理想体重的135%以上）。他们的VLDL-TG水平各不相同，从正常到显著升高。在许多受试者中，VLDL的浓度与转运之间存在正相关，表明VLDL-TG的过度产生导致了高甘油三酯血症。在其他受试者中，特别是在一些显著肥胖的受试者中，转运速率大幅增加而无明显的高甘油三酯血症，表明他们清除TG的能力增强。然而，在所有组中，都有一些患者的高甘油三酯血症程度似乎与他们的转运速率不成比例。这一发现以及许多患者VLDL-TG分泌增加而血浆TG未升高的事实表明，VLDL-TG的过度产生和清除增强不足都促成了高甘油三酯血症的发展。数据显示，我们的多室分析测定的转运速率与其他研究者先前使用的单指数分析之间相关性较差（r = 0.46）；根据肥胖程度对患者进行分类并不能改善这种比较。尽管需要两种转化途径（快速和缓慢合成途径）来拟合数据，但转运速率与两种途径的比例之间没有相关性。此外，尽管已知VLDL转化为低密度脂蛋白的途径，但未发现VLDL-TG转运速率与估计的低密度脂蛋白胆固醇浓度之间存在相关性。
