School of Anatomy, Physiology and Human Biology, The University of Western Australia, Perth 6009, Australia.
School of Chemistry and Biochemistry, The University of Western Australia, Perth 6009, Australia.
Nutrients. 2016 Nov 8;8(11):711. doi: 10.3390/nu8110711.
Human milk (HM) contains a plethora of metabolic hormones, including leptin, which is thought to participate in the regulation of the appetite of the developing infant. Leptin in HM is derived from a combination of de novo mammary synthesis and transfer from the maternal serum. Moreover, leptin is partially lipophilic and is also present in HM cells. However, leptin has predominately been measured in skim HM, which contains neither fat nor cells. We optimised an enzyme-linked immunosorbent assay for leptin measurement in both whole and skim HM and compared leptin levels between both HM preparations collected from 61 lactating mothers. Whole HM leptin ranged from 0.2 to 1.47 ng/mL, whilst skim HM leptin ranged from 0.19 to 0.9 ng/mL. Whole HM contained, on average, 0.24 ± 0.01 ng/mL more leptin than skim HM ( < 0.0001, = 287). No association was found between whole HM leptin and fat content ( = 0.17, = 287), supporting a cellular contribution to HM leptin. No difference was found between pre- and post-feed samples (whole HM: = 0.29, skim HM: = 0.89). These findings highlight the importance of optimising HM leptin measurement and assaying it in whole HM to accurately examine the amount of leptin received by the infant during breastfeeding.
人乳（HM）中含有大量代谢激素，包括瘦素，瘦素被认为参与调节婴儿的食欲。HM 中的瘦素来源于乳腺的从头合成和母体血清的转移。此外，瘦素部分具有亲脂性，也存在于 HM 细胞中。然而，瘦素主要在脱脂 HM 中进行测量，脱脂 HM 既不含脂肪也不含细胞。我们优化了一种用于测量全脂和脱脂 HM 中瘦素的酶联免疫吸附测定法，并比较了 61 位哺乳期母亲采集的两种 HM 制剂中的瘦素水平。全脂 HM 中的瘦素浓度范围为 0.2 至 1.47ng/ml，而脱脂 HM 中的瘦素浓度范围为 0.19 至 0.9ng/ml。全脂 HM 平均比脱脂 HM 多含有 0.24 ± 0.01ng/ml 的瘦素（<0.0001，=287）。全脂 HM 中的瘦素与脂肪含量之间没有相关性（=0.17，=287），这支持了 HM 瘦素的细胞贡献。在喂养前后的样本中未发现差异（全脂 HM：=0.29，脱脂 HM：=0.89）。这些发现强调了优化 HM 瘦素测量和在全脂 HM 中进行检测的重要性，以便准确地检查婴儿在母乳喂养期间接收到的瘦素量。
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