Interdisciplinary Program, Bioengineering Major, Graduate School, Seoul National University, Seoul 110-744, Republic of Korea.
Biosens Bioelectron. 2012 Jan 15;31(1):284-91. doi: 10.1016/j.bios.2011.10.033. Epub 2011 Oct 24.
This paper describes the first devised method for the nonenzymatic and electrochemical glucose monitoring in 100% human whole blood and serum. The nanoporous Pt electrode allows for the selective amplification of glucose oxidation in the presence of electroactive interfering species without the need for enzymatic reaction. The outer membrane was particularly optimized to allow glucose molecules to be electrochemically detected against the numerous constituents of human blood. The proposed sensor provided reproducible amperometric responses to glucose in human serum and whole blood. Its sensitivity was maintained for at least 7h under constantly electrified conditions, and continued to work properly after 30 days of storage in human whole blood and serum. Unlike the enzyme-based glucose sensors, it was found to be minimally affected by thermal fluctuation, so as to remain successful even after steam sterilization at high temperature (134 °C) and pressure (0.22 Mpa). The unprecedented long-term stability and sterilization compatibility observed herein suggest a promising alternative to conventional enzymatic glucose sensors for many analytical and clinical applications, particularly for continuous glucose monitoring devices designed to potentially lead to a closed-loop artificial pancreas by combining them with an insulin pump.
本文描述了一种在 100%人全血和血清中进行非酶和电化学葡萄糖监测的首创方法。纳米多孔 Pt 电极允许在存在电活性干扰物质的情况下选择性放大葡萄糖氧化，而无需酶反应。外膜经过特别优化，允许电化学检测葡萄糖，同时对抗人体血液中的众多成分。所提出的传感器在人血清和全血中对葡萄糖提供了可重复的电流响应。在持续通电条件下，其灵敏度至少可保持 7 小时，在人全血和血清中储存 30 天后仍能正常工作。与基于酶的葡萄糖传感器不同，它受温度波动的影响极小，因此即使在高温（134°C）和高压（0.22Mpa）下进行蒸汽灭菌后，仍能成功工作。本文观察到前所未有的长期稳定性和灭菌兼容性，为许多分析和临床应用提供了一种有前途的替代传统酶葡萄糖传感器的方法，特别是对于与胰岛素泵结合使用以潜在实现闭环人工胰腺的连续葡萄糖监测设备。
