大脑与免疫系统：完整的免疫系统对于阿片类成瘾大鼠戒断症状的表现至关重要。

The brain and the immune system: an intact immune system is essential for the manifestation of withdrawal in opiate addicted rats.

作者信息

Dougherty P M, Pellis N R, Dafny N

机构信息

Department of Neurobiology and Anatomy, University of Texas Medical School Houston 77225.

出版信息

Neuroscience. 1990;36(2):285-9. doi: 10.1016/0306-4522(90)90293-d.

DOI:10.1016/0306-4522(90)90293-d

PMID:2215925

Abstract

Gamma irradiation (500 rad) is often used to suppress the immune system in mice, rats and man. Recently, it was shown that irradiation prior to chronic morphine treatment, dramatically reduces the severity of naloxone-precipitated withdrawal in morphine-dependent animals. In the present study adoptive transfer of 2-6 x 10(8) splenocytes to irradiated rats prior to chronic morphine treatment restored the severity of all withdrawal signs precipitated by naloxone. In contrast, adoptive transfer of fractionated splenocyte subpopulations only partially restored withdrawal severity; and transfer of irradiated splenocytes, red blood cells or diluted numbers of normal splenocytes did not have any observed restorative effect. These findings suggest that specific cellular activities or factors derived from lymphoid cells are required for the expression of opiate withdrawal.

摘要

γ射线照射（500拉德）常用于抑制小鼠、大鼠和人类的免疫系统。最近有研究表明，在慢性吗啡治疗前进行照射，可显著减轻吗啡依赖动物中纳洛酮诱发的戒断症状的严重程度。在本研究中，在慢性吗啡治疗前将2-6×10⁸个脾细胞过继转移到受照射的大鼠体内，可恢复纳洛酮诱发的所有戒断症状的严重程度。相比之下，分馏的脾细胞亚群过继转移仅部分恢复了戒断严重程度；而照射过的脾细胞、红细胞或稀释数量的正常脾细胞的转移未观察到任何恢复作用。这些发现表明，阿片类药物戒断症状的表达需要淋巴细胞衍生的特定细胞活性或因子。