Department of Orthodontics, Kyung-Hee University College of Dental Medicine, Seoul 130-701, Republic of Korea.
Biochem Biophys Res Commun. 2012 Jan 13;417(2):697-703. doi: 10.1016/j.bbrc.2011.11.157. Epub 2011 Dec 7.
Special AT-rich sequence-binding protein (SATB) plays a critical role in bone generation and osteoblast differentiation. In the present study, the differentially expressed genes by SATB2 overexpression were analyzed in MC3T3-E1 osteoblast-like cells using Alizarin red S staining, wound healing assay and Agilent's Human Oligo Microarray. Calcium mineralization and motility were significantly enhanced in SATB2-overexpressed cells compared with untreated control. In addition, using the GeneSpringGX 7.3 program to compare the identified genes expressed in SATB2-overexpresed cells with untreated control, we found several unique genes closely associated with osteoblast differentiation, including SOX2, MBP2, WNT11 and MEN1 (up-regulated genes), and ILK, FGF23, FGFR2, and SNAI1 (down-regulated genes). Consistent with microarray data, real-time RT-PCR confirmed the significant up- and down-regulation of these genes at mRNA level in SATB2-overexpressed MC3T3-E1 cells. Overall, our findings suggest that the molecular regulation of SATB2 can be an attractive approach to develop a novel therapeutic strategy for bone-related diseases.
特异性 AT 富含序列结合蛋白（SATB）在骨骼生成和成骨细胞分化中起着关键作用。在本研究中，使用茜素红 S 染色、划痕愈合试验和安捷伦的 Human Oligo Microarray 分析了 SATB2 过表达对 MC3T3-E1 成骨样细胞的差异表达基因。与未处理的对照组相比，SATB2 过表达细胞的钙矿化和迁移能力显著增强。此外，使用 GeneSpringGX 7.3 程序比较 SATB2 过表达细胞与未处理对照组中表达的鉴定基因，我们发现了几个与成骨细胞分化密切相关的独特基因，包括 SOX2、MBP2、WNT11 和 MEN1（上调基因），以及 ILK、FGF23、FGFR2 和 SNAI1（下调基因）。与微阵列数据一致，实时 RT-PCR 证实了 SATB2 过表达 MC3T3-E1 细胞中这些基因在 mRNA 水平的显著上调和下调。总的来说，我们的研究结果表明，SATB2 的分子调控可能成为开发与骨骼相关疾病的新型治疗策略的有吸引力的方法。
