Wongkajornsilp Adisak, Sa-Ngiamsuntorn Khanit, Hongeng Suradej
Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Methods Mol Biol. 2012;826:73-87. doi: 10.1007/978-1-61779-468-1_7.
Clones of hepatocyte-like cells were reproducibly generated from human mesenchymal stem cells immortalized with a combined transduction of both Bmi-1 and TERT genes. These hepatocyte-like cells contained selective markers and several functional properties of hepatocytes, yet still carried proliferative potential. These cells had cuboidal morphology and arranged themselves as cord-like structure in culture. The cloned cells deposited glycogen and actively synthesized albumin. The basal expressions of CYP450 isozymes was observed, albeit only 10-20% that of primary hepatocytes. These expressions were promptly increased upon the addition of rifampicin, a known enzyme inducer. These hepatocyte-like cells may serve as a close alternative to the use of primary hepatocytes for in vitro studies.
通过同时转导Bmi-1和TERT基因而永生化的人间充质干细胞可重复地产生肝细胞样细胞克隆。这些肝细胞样细胞含有肝细胞的选择性标志物和几种功能特性，但仍具有增殖潜力。这些细胞呈立方形形态，在培养物中排列成索状结构。克隆细胞储存糖原并积极合成白蛋白。观察到CYP450同工酶的基础表达，尽管仅为原代肝细胞的10%-20%。加入已知的酶诱导剂利福平后，这些表达迅速增加。这些肝细胞样细胞可作为原代肝细胞用于体外研究的一种相近替代物。
