Israel M A, Chan H W, Hourihan S L, Rowe W P, Martin M A
J Virol. 1979 Mar;29(3):990-6. doi: 10.1128/JVI.29.3.990-996.1979.
The biological activity of polyoma viral DNA was evaluated in mice and hamsters. Viral DNA administered parenterally is about 4 to 5 logs less efficient than polyoma virions in establishing infection in mice. Supercoiled viral DNA was infectious for mice after parenteral administration, giving mean infective doses of 10(-3) to 10(-4) microgram. However, animals fed microgram quantities of polyoma DNA I did not become infected. Linearization of viral DNA with R.EcoRI or R.BamHI, which are single-cut enzymes cleaving in the early and late regions of the genome, respectively, reduced the infectivity for mice approximately fivefold. Approximately 10% of newborn hamsters inoculated intraperitoneally with polyoma DNA I developed tumors. In contrast, the same amount of viral DNA which had been cleaved in the early region with R.EcoRI induced tumors in 50% of inoculated hamsters.
在小鼠和仓鼠体内评估了多瘤病毒DNA的生物活性。经肠胃外给药的病毒DNA在小鼠体内建立感染的效率比多瘤病毒颗粒低约4至5个对数。超螺旋病毒DNA经肠胃外给药后对小鼠具有感染性，平均感染剂量为10^(-3)至10^(-4)微克。然而，喂食微克量多瘤DNA I的动物并未被感染。用R.EcoRI或R.BamHI使病毒DNA线性化，这两种酶分别是在基因组早期和晚期区域切割的单一切割酶，使对小鼠的感染性降低了约五倍。约10%经腹腔接种多瘤DNA I的新生仓鼠发生了肿瘤。相比之下，用R.EcoRI在早期区域切割的相同量病毒DNA在50%的接种仓鼠中诱发了肿瘤。
