Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002 U.P., India.
J Hazard Mater. 2012 Jan 30;201-202:92-9. doi: 10.1016/j.jhazmat.2011.11.044. Epub 2011 Nov 22.
The work described here presents the toxic effect of Mathura refinery wastewater (MRWW) in plant (Allium cepa), bacterial (E. coli K12) and human (blood) system. The samples were collected from adjoining area of Mathura refinery, Dist. Mathura, U.P. (India). Chromosomal aberration test and micronucleus assay in (A. cepa) system, E. coli K12 survival assay as well as hemolysis assay in human blood were employed to assess the toxicity of MRWW. MRWW exposure resulted in the formation of micronuclei and bridges in chromosomes of A. cepa cells. A significant decline occurred in survival of DNA repair defective mutants of E. coli K12 exposed to MRWW. On incubation with MRWW, calf thymus DNA-EtBr fluorescence intensity decreased and percent hemolysis of human blood cells increased. An induction in the MDA levels of MRWW treated A. cepa roots indicated lipid peroxidation also. Collectively, the results demonstrate a significant genotoxic and cytotoxic potential of MRWW.
这里描述的工作展示了马图拉精炼厂废水 (MRWW) 对植物（洋葱）、细菌（大肠杆菌 K12）和人体（血液）系统的毒性作用。这些样本是从印度北方邦马图拉地区毗邻的马图拉精炼厂采集的。采用洋葱染色体畸变试验和微核试验、大肠杆菌 K12 生存试验和人血溶血试验来评估 MRWW 的毒性。MRWW 暴露导致洋葱细胞染色体形成微核和桥。暴露于 MRWW 的 DNA 修复缺陷型大肠杆菌 K12 的生存能力显著下降。与人血孵育时，小牛胸腺 DNA-EtBr 荧光强度降低，人血细胞的溶血率增加。用 MRWW 处理的洋葱根尖 MDA 水平的升高表明也发生了脂质过氧化。总的来说，这些结果表明 MRWW 具有显著的遗传毒性和细胞毒性潜力。
