Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing, China.
Cell Prolif. 2012 Feb;45(1):15-21. doi: 10.1111/j.1365-2184.2011.00800.x. Epub 2011 Dec 16.
Recently, plant lectins have attracted great interest due to their various biological activities such as anti-cancer, anti-fungal and anti-viral activities. We have reported earlier concerning anti-proliferation of human cancer cell lines by a galactose-binding lectin (AML), from a Chinese herb, ASTRAGALUS MEMBRANACEUS: In the present study, detailed investigations into the mechanism of such anti-proliferation properties have been carried out.
Mechanism of apoptosis initiation in K562 cells by AML was investigated by morphology, flow cytometry and western blot analysis.
AML induced apoptosis in a caspase-dependent manner in the chronic myeloid leukemia cell line, K562. Furthermore, we observed that cytotoxicity and apoptosis of K562 cells induced by AML were completely abolished in presence of lactose or galactose.
Our results suggest that AML could act as a potential anti-cancer drug.
由于植物凝集素具有多种生物活性，如抗癌、抗真菌和抗病毒活性，因此近来引起了极大的关注。我们之前曾报道过一种来自中药黄芪的半乳糖结合凝集素（AML）对人癌细胞系的增殖具有抑制作用：在本研究中，我们对这种抗增殖特性的机制进行了详细的研究。
通过形态学、流式细胞术和 Western blot 分析研究 AML 在 K562 细胞中诱导细胞凋亡的机制。
AML 以半胱天冬酶依赖性方式诱导慢性髓系白血病细胞系 K562 发生细胞凋亡。此外，我们观察到 AML 诱导的 K562 细胞的细胞毒性和细胞凋亡在乳糖或半乳糖存在的情况下完全被抑制。
我们的结果表明，AML 可能是一种潜在的抗癌药物。
