Wang Yuefei, Sun Da, Chen Hao, Qian Lisheng, Xu Ping
Department of Tea Science, Zhejiang University, Hangzhou 310058, China; E-Mails:
Int J Mol Sci. 2011;12(11):7708-19. doi: 10.3390/ijms12117708. Epub 2011 Nov 8.
Seeds are another product in addition to leaves (raw materials for teas) of tea (Camellia sinensis L.) plant. The great increase of tea consumption in recent years raises the challenge of finding commercial applications for tea seeds. In the present study, supercritical carbon dioxide (SC-CO(2)) extraction edible oil from tea seed was carried out, response surface methodology (RSM) was used to optimize processing parameters including time (20-90 min), temperature (35-45 °C) and pressure (50-90 MPa). The fatty acid composition and antioxidant activity of the extracted oil was also investigated. The highest yield of oil (29.2 ± 0.6%) was obtained under optimal SC-CO(2) extraction conditions (45 °C, 89.7 min and 32 MPa, respectively), which was significantly higher (p < 0.05) than that (25.3 ± 1.0%) given by Soxhlet extraction. Meanwhile, tea seed oil extracted by SC-CO(2) contained approximately 80% unsaturated fatty acids and showed a much stronger scavenging ability on the DPPH radical than that extracted by Soxhlet. SC-CO(2) is a promising alternative for efficient extraction of edible oil from tea seed. Moreover, tea seed oil extracted by SC-CO(2) is highly edible and has good antioxidant activity, and therefore may play a potential role as a health-promoting food resource in human diets.
种子是茶树(Camellia sinensis L.)除叶子(茶叶原料)之外的另一产物。近年来茶叶消费量的大幅增长对寻找茶籽的商业用途提出了挑战。在本研究中,采用超临界二氧化碳(SC-CO₂)从茶籽中提取食用油,并运用响应面法(RSM)优化包括时间(20 - 90分钟)、温度(35 - 45℃)和压力(50 - 90兆帕)在内的工艺参数。同时还研究了提取油的脂肪酸组成和抗氧化活性。在最佳SC-CO₂提取条件(分别为45℃、89.7分钟和32兆帕)下,油的最高得率为(29.2±0.6%),显著高于索氏提取法的得率(25.3±1.0%)(p<0.05)。此外,SC-CO₂提取的茶籽油含有约80%的不饱和脂肪酸,对DPPH自由基的清除能力远强于索氏提取法提取的茶籽油。SC-CO₂是从茶籽中高效提取食用油的一种有前景的替代方法。而且,SC-CO₂提取的茶籽油具有高度可食用性且抗氧化活性良好,因此可能在人类饮食中作为一种促进健康的食品资源发挥潜在作用。