Pharmacy College of Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China.
Molecules. 2011 Dec 5;16(12):10029-45. doi: 10.3390/molecules161210029.
The supercritical fluid extraction (SFE) of Lepidium apetalum seed oil and its anti-oxidant activity were studied. The SFE process was optimized using response surface methodology (RSM) with a central composite design (CCD). Independent variables, namely operating pressure, temperature, time and flow rate were evaluated. The maximum extraction of Lepidium apetalum seed oil by SFE-CO₂ (about 36.3%) was obtained when SFE-CO₂ extraction was carried out under the optimal conditions of 30.0 MPa of pressure, 70 °C of temperature, 120 min of extraction time and 25.95 L/h of flow rate. GC-MS analysis showed the presence of four fatty acids in Lepidium apetalum seed oil, with a high content (91.0%) of unsaturated fatty acid. The anti-oxidant activity of the oil was assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay and 2,2'-azino- bis(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS) test. Lepidium apetalum seed oil possessed a notable concentration-dependent antioxidant activity, with IC₅₀ values of 1.00 and 3.75 mg/mL, respectively.
超临界流体萃取（SFE）法萃取芝麻菜籽油及其抗氧化活性的研究。采用中心复合设计（CCD）的响应面法（RSM）对 SFE 过程进行了优化。独立变量，即操作压力、温度、时间和流速进行了评估。当 SFE-CO₂在 30.0 MPa 的压力、70°C 的温度、120 min 的提取时间和 25.95 L/h 的流速的最佳条件下进行时，通过 SFE-CO₂萃取获得了芝麻菜籽油的最大提取量（约 36.3%）。GC-MS 分析表明，芝麻菜籽油中存在四种脂肪酸，其中不饱和脂肪酸含量（91.0%）较高。通过 2,2-二苯基-1-苦基肼（DPPH）自由基清除测定法和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）试验评估了油的抗氧化活性。芝麻菜籽油具有显著的浓度依赖性抗氧化活性，IC₅₀值分别为 1.00 和 3.75 mg/mL。
