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3,3'-二乙基硫代三碳菁碘化物：一种用于检测DNA螺旋度和序列的高灵敏度手性光学报告分子。

3,3'-diethylthiatricarbocyanine iodide: a highly sensitive chiroptical reporter of DNA helicity and sequence.

作者信息

Choi Jung Kyu, D'Urso Alessandro, Trauernicht Mitch, Shabbir-Hussain Murtaza, Holmes Andrea E, Balaz Milan

机构信息

Department of Chemistry, University of Wyoming, Laramie, WY 82071, USA; E-Mails:

出版信息

Int J Mol Sci. 2011;12(11):8052-62. doi: 10.3390/ijms12118052. Epub 2011 Nov 16.

DOI:10.3390/ijms12118052
PMID:22174649
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3233455/
Abstract

Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3'-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)(2) and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal.

摘要

利用紫外可见吸收光谱和圆二色（CD）光谱，我们研究了3,3'-二乙基硫代三碳菁碘化物（Cy7）与不同序列和螺旋结构的多核苷酸之间的结合相互作用。CD显示出是一种非常有诊断价值的工具，在Cy7结合后，对于所有研究的DNA序列都给出了不同的光谱手性光学特征。Cy7能够通过几乎相反的诱导圆二色信号，在光谱上区分聚（dG-dC）(2)的右手B-DNA及其由精胺或六胺钴（III）诱导的左手Z-DNA对应物。

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