在生理离子强度条件下识别未修饰的短寡核苷酸的左手 Z-DNA。
Recognition of left-handed Z-DNA of short unmodified oligonucleotides under physiological ionic strength conditions.
机构信息
Department of Chemistry, University of Wyoming, Laramie, WY 82071, USA.
出版信息
Biochem Biophys Res Commun. 2010 Jun 25;397(2):329-32. doi: 10.1016/j.bbrc.2010.05.119. Epub 2010 May 27.
Abstract
The left-handed Z-DNA form of the short unmodified alternating guanine-cytosine oligonucleotides, 5'-(dGdC)(24) and 5'-(dGdC)(18), was selectively detected under physiological ionic strength and pH conditions using the anionic nickel(II) porphyrin, NiTPPS. No spectroscopic signal was observed for NiTPPS with any right-handed oligonucleotides under identical conditions. The 48mer 5'-(dGdC)(24) Z-form was detected at concentrations as low as 100nM. The binding of NiTPPS to the B- and Z-oligonucleotides was studied quantitatively by UV-vis absorption and circular dichroism spectroscopies. NiTPPS was found to be a universal DNA binder, with binding affinity and geometry depending on the ionic composition of the solution, rather than on the DNA helical twist. This is the first example of a successful spectroscopic detection of the Z-DNA of short unmodified oligonucleotides under physiological pH and ionic strength conditions.
摘要
左手 Z-DNA 形式的短未修饰的交替鸟嘌呤-胞嘧啶寡核苷酸，5'-(dGdC)(24) 和 5'-(dGdC)(18)，在生理离子强度和 pH 条件下，使用阴离子镍(II)卟啉 NiTPPS 可选择性检测到。在相同条件下，任何右手寡核苷酸与 NiTPPS 均未观察到光谱信号。48mer 5'-(dGdC)(24)Z 型在低至 100nM 的浓度下即可检测到。通过紫外-可见吸收和圆二色性光谱研究了 NiTPPS 与 B-和 Z-寡核苷酸的结合。发现 NiTPPS 是一种通用的 DNA 结合剂，其结合亲和力和几何形状取决于溶液的离子组成，而不是 DNA 螺旋扭曲。这是在生理 pH 和离子强度条件下成功检测短未修饰寡核苷酸 Z-DNA 的首例实例。
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