The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Fitoterapia. 2012 Mar;83(2):388-94. doi: 10.1016/j.fitote.2011.12.001. Epub 2011 Dec 9.
Antitumor activities of water extract (fraction A), ethanol extract (fraction B), ethyl acetate extract (fraction C), non-ethyl acetate extract (fraction D) and compound diosbulbin B isolated from Dioscorea bulbifera L. (DB) were investigated in vivo in this present study. The results showed that fractions B and C both decreased tumor weight in S180 and H22 tumor cells bearing mice, while fractions A and D had no such effect. Furthermore, fraction C altered the weight of spleen and thymus, and the amounts of total leukocytes, lymphocytes and neutrophils in tumor-bearing mice. Further results showed that compound diosbulbin B demonstrated anti-tumor effects in the dose-dependent manner at the dosage of 2 to 16 mg/kg without significant toxicity in vivo. Furthermore, on the basis of chemical analysis of the above extracts by high-performance liquid chromatography (HPLC) with a diode array detector (DAD), diosbulbin B was found to be the major antitumor bioactive component of DB. These results suggest that DB has potential anti-tumor effects which may be related to influencing the immune system for the first time, and the compound diosbulbin B is the major antitumor component of DB.
本研究考察了从穿龙薯蓣（DB）中分离得到的水提取物（A 部分）、乙醇提取物（B 部分）、乙酸乙酯提取物（C 部分）、非乙酸乙酯提取物（D 部分）和化合物薯蓣皂素 B（B 部分）的体内抗肿瘤活性。结果表明，B 部分和 C 部分均能降低 S180 和 H22 荷瘤小鼠的肿瘤重量，而 A 部分和 D 部分则没有这种作用。此外，C 部分改变了荷瘤小鼠脾脏和胸腺的重量以及总白细胞、淋巴细胞和中性粒细胞的数量。进一步的结果表明，化合物薯蓣皂素 B 在 2 至 16mg/kg 剂量范围内具有剂量依赖性的抗肿瘤作用，体内无明显毒性。此外，基于高效液相色谱（HPLC）与二极管阵列检测器（DAD）对上述提取物的化学分析，发现薯蓣皂素 B 是 DB 的主要抗肿瘤生物活性成分。这些结果表明，DB 具有潜在的抗肿瘤作用，这可能是首次与影响免疫系统有关，化合物薯蓣皂素 B 是 DB 的主要抗肿瘤成分。
