Liu Ziqi, Zhong Lian, Wang Lingyu, Li Meiyan, Chen Chao
School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, PR China.
Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Scienceand Technology of China, Chengdu 610072, P.R. China.
Heliyon. 2023 Aug 6;9(8):e18886. doi: 10.1016/j.heliyon.2023.e18886. eCollection 2023 Aug.
Thyroid cancer (TC), a common endocrine malignant tumor with a higher incidence in females than in males, is a serious threat to human life and health. Although current clinical treatments can alleviate this disease, the recurrence rate remains high. Tuber , also called (HYZ), has remarkable curative properties, few side effects and is used for the treatment of sore throat, goiter, hemoptysis, and other diseases in traditional Chinese medicine (TCM). Existing clinical studies have found that HYZ can improve the clinical symptoms of TC patients and reduce tumor volume, while in vitro studies have found that HYZ can induce the death of thyroid cancer cells. However, the mechanism of HYZ in the treatment of TC is still unclear.
In this study, based on network pharmacology and bioinformatics, the target and molecular mechanism of HYZ in the treatment of TC were preliminarily explored. The results suggest that the antithyroid cancer effect of HYZ may be related to the PI3K-Akt and focal adhesion pathways. Then, a TC cell model was established to further explore the detail molecular mechanisms.
Fortunately, HYZ induced apoptosis in KMH-2 cells and regulated the expression of apoptosis-related proteins and genes. At the same time, HYZ can also significantly inhibit the migration and invasion of TC cells. Further studies showed that the pharmacological activities of HYZ were related to the regulation of the PI3K-Akt and focal adhesion pathways.
Our study provides a reference for further animal or clinical studies investigating the effectiveness and molecular mechanisms of HYZ against thyroid cancer.
甲状腺癌（TC）是一种常见的内分泌恶性肿瘤，女性发病率高于男性，严重威胁人类生命健康。尽管目前的临床治疗可以缓解这种疾病，但复发率仍然很高。土贝母，也称为（HZ），具有显著的治疗特性，副作用少，在中医中用于治疗咽喉肿痛、甲状腺肿、咯血等疾病。现有临床研究发现，HZ可以改善TC患者的临床症状并缩小肿瘤体积，而体外研究发现，HZ可以诱导甲状腺癌细胞死亡。然而，HZ治疗TC的机制仍不清楚。
在本研究中，基于网络药理学和生物信息学，初步探索了HZ治疗TC的靶点和分子机制。结果表明，HZ的抗甲状腺癌作用可能与PI3K-Akt和粘着斑途径有关。然后，建立了TC细胞模型以进一步探索详细的分子机制。
幸运的是，HZ诱导KMH-2细胞凋亡并调节凋亡相关蛋白和基因的表达。同时，HZ还可以显著抑制TC细胞的迁移和侵袭。进一步研究表明，HZ的药理活性与PI3K-Akt和粘着斑途径的调节有关。
我们的研究为进一步研究HZ抗甲状腺癌的有效性和分子机制的动物或临床研究提供了参考。
