Center for Molecular Imaging, The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
Technol Cancer Res Treat. 2012 Feb;11(1):95-104. doi: 10.7785/tcrt.2012.500238.
With the aid of indocyanine green (ICG), lymphatic architecture and function in both mice and humans has been successfully imaged non-invasively using near-infrared (NIR) fluorescence imaging devices. Maximal measurement sensitivity of NIR fluorescence imaging devices is needed for "first-in-humans" molecularly targeting NIR fluorescence agents that are brighter than non-specific ICG. In this study, we developed a solid phantom and measurement approach for the quantification of excitation light leakage and measurement sensitivity of NIR fluorescence imaging devices. The constructed solid phantom, consisting of quantum dots impregnated onto specularly reflective surface, shows long-term stability and can be used as a traceable fluorescence standard. With the constructed solid phantom, the intensified CCD (ICCD)-based device demonstrated more than 300% higher measurement sensitivity compared to the Electron Multiplying CCD (EMCCD) based device when integration time was maintained less than 1.0 s.
借助吲哚菁绿（ICG），已经成功地使用近红外（NIR）荧光成像设备对小鼠和人类的淋巴结构和功能进行了非侵入性成像。对于“首次在人体中”进行分子靶向 NIR 荧光试剂的检测，需要 NIR 荧光成像设备具有最大的测量灵敏度，因为这些试剂比非特异性 ICG 更亮。在这项研究中，我们开发了一种固态幻像和测量方法，用于量化激发光泄漏和 NIR 荧光成像设备的测量灵敏度。所构建的固态幻像由量子点浸渍在镜面反射表面上组成，具有长期稳定性，可作为可追踪的荧光标准。使用构建的固态幻像，当积分时间保持在 1.0 秒以下时，基于增强型 CCD（ICCD）的设备与基于电子倍增 CCD（EMCCD）的设备相比，测量灵敏度提高了 300%以上。
