Applied Physical Chemistry, Ruprecht-Karls-University Heidelberg, Germany.
Phys Rev Lett. 2011 Nov 11;107(20):208101. doi: 10.1103/PhysRevLett.107.208101. Epub 2011 Nov 8.
The unique strengths of x-ray microscopy are high penetration depth and near-edge resonances that provide chemical information. We use ptychography, a coherent diffractive imaging technique that disposes of the requirement for isolated specimens, and demonstrate resonant imaging by exploiting resonances near the oxygen K edge to differentiate between two oxygen-containing materials. To highlight a biological system where resonant ptychography might be used for chemical mapping of unsliced cells, reconstructions of freeze-dried Deinococcus radiodurans cells at an energy of 517 eV are shown.
X 射线显微镜的独特优势在于其具有较高的穿透深度和近边共振，可提供化学信息。我们使用相衬衍射成像技术（一种不需要单独样本的相干衍射成像技术），并利用氧 K 边附近的共振来区分两种含氧材料，从而展示了共振相衬成像的效果。为了突出展示共振相衬可能用于未经切片的细胞化学映射的生物系统，我们展示了在 517eV 能量下对冻干的放射性球菌细胞的重建。
