Programa de Pós-Graduação em Neurociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Braz J Med Biol Res. 2012 Jan;45(1):49-57. doi: 10.1590/s0100-879x2011007500162. Epub 2011 Dec 23.
Cell transplantation is a promising experimental treatment for spinal cord injury. The aim of the present study was to evaluate the efficacy of mononuclear cells from human umbilical cord blood in promoting functional recovery when transplanted after a contusion spinal cord injury. Female Wistar rats (12 weeks old) were submitted to spinal injury with a MASCIS impactor and divided into 4 groups: control, surgical control, spinal cord injury, and one cell-treated lesion group. Mononuclear cells from umbilical cord blood of human male neonates were transplanted in two experiments: a) 1 h after surgery, into the injury site at a concentration of 5 x 10(6) cells diluted in 10 µL 0.9% NaCl (N = 8-10 per group); b) into the cisterna magna, 9 days after lesion at a concentration of 5 x 10(6) cells diluted in 150 µL 0.9% NaCl (N = 12-14 per group). The transplanted animals were immunosuppressed with cyclosporin-A (10 mg/kg per day). The BBB scale was used to evaluate motor behavior and the injury site was analyzed with immunofluorescent markers to label human transplanted cells, oligodendrocytes, neurons, and astrocytes. Spinal cord injury rats had 25% loss of cord tissue and cell treatment did not affect lesion extension. Transplanted cells survived in the injured area for 6 weeks after the procedure and both transplanted groups showed better motor recovery than the untreated ones (P < 0.05). The transplantation of mononuclear cells from human umbilical cord blood promoted functional recovery with no evidence of cell differentiation.
细胞移植是一种有前途的脊髓损伤实验治疗方法。本研究的目的是评估人脐血单个核细胞在挫伤性脊髓损伤后移植时促进功能恢复的疗效。雌性 Wistar 大鼠（12 周龄）用 MASCIS 撞击器进行脊髓损伤，分为 4 组：对照组、手术对照组、脊髓损伤组和 1 个细胞治疗损伤组。将来自男性新生儿脐带血的单个核细胞在两项实验中进行移植：a）手术后 1 小时，以 5 x 10(6)个细胞的浓度稀释在 10 µL 0.9% NaCl 中（每组 8-10 只）；b）在损伤后第 9 天，以 5 x 10(6)个细胞的浓度稀释在 150 µL 0.9% NaCl 中（每组 12-14 只），移植到鞘内。移植动物用环孢素 A（每天 10 mg/kg）进行免疫抑制。BBB 量表用于评估运动行为，并用免疫荧光标志物分析损伤部位，以标记人移植细胞、少突胶质细胞、神经元和星形胶质细胞。脊髓损伤大鼠的脊髓组织损失了 25%，细胞治疗并未影响损伤的扩展。移植细胞在手术后 6 周内在损伤区域存活，且两个移植组的运动恢复均优于未治疗组（P < 0.05）。人脐血单个核细胞的移植促进了功能恢复，没有细胞分化的证据。
