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小鼠脊髓损伤后即刻及7天时单剂量脐血来源单个核细胞注射的评估

Evaluation of single-dose umbilical cord blood-derived mononuclear cell injection immediately and 7 days after spinal cord trauma in mice.

作者信息

de Araujo Alex Oliveira, Dos Santos Gustavo Bispo, Marcon Raphael Martus, Nicola Maria Helena Alves, Pereira Marcela Saldanha, Sanchez Fernando Barbosa, Araujo Thiego Pedro Freitas, Cristante Alexandre Fogaça

机构信息

Department of Orthopedic Surgery, Rede SARAH de Hospitais de Reabilitação, (SMHS), Brasília, DF, Brazil.

Department of Orthopedic Surgery, Instituto de Ortopedia e Traumatologia da Universidade de São Paulo, São Paulo, SP, Brazil.

出版信息

Clinics (Sao Paulo). 2025 Jan 30;80:100579. doi: 10.1016/j.clinsp.2025.100579. eCollection 2025.

DOI:10.1016/j.clinsp.2025.100579
PMID:39889361
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11833352/
Abstract

STUDY DESIGN

Experimental study utilizing a standardized Balb C mouse model.

OBJECTIVES

Evaluate histological changes and motor function recovery in the acute and subacute phases of Spinal Cord Injury (SCI) in mice using human Umbilical cord blood-derived mononuclear cells.

METHODS

Forty mice were divided into five groups, with two receiving human Umbilical cord blood-derived mononuclear cells immediately after SCI and after 7 days, and three control groups. Motor assessment utilized BMS, MFS, and horizontal plane scales over six weeks. Necropsy evaluated macroscopic and histological spinal cord changes.

RESULTS

Histologically, Umbilical cord blood-derived mononuclear cells-treated groups exhibited significant reductions in necrosis, hemorrhage, and degeneration compared to controls. Motor recovery showed partial improvement across all groups, with no statistically significant differences in scales between intervention and control groups.

CONCLUSIONS

In the acute phases of SCI, Umbilical cord blood-derived mononuclear cells applied directly to Balb C mice lesions demonstrated histological improvement but played a limited role in functional enhancement. The study highlights distinctions in the treatment's efficacy, potentially related to these cells' diverse differentiation capacities and intrinsic properties.

摘要

研究设计

采用标准化的Balb C小鼠模型进行实验研究。

目的

使用人脐带血来源的单核细胞评估小鼠脊髓损伤（SCI）急性和亚急性期的组织学变化及运动功能恢复情况。

方法

将40只小鼠分为五组，其中两组在脊髓损伤后立即及7天后接受人脐带血来源的单核细胞，另外三组为对照组。在六周内使用BMS、MFS和水平面量表进行运动评估。尸检评估脊髓的宏观和组织学变化。

结果

组织学上，与对照组相比，脐带血来源的单核细胞治疗组的坏死、出血和变性明显减少。所有组的运动恢复均有部分改善，干预组和对照组在量表上无统计学显著差异。

结论

在脊髓损伤的急性期，直接应用于人Balb C小鼠损伤部位的脐带血来源的单核细胞显示出组织学改善，但在功能增强方面作用有限。该研究突出了治疗效果的差异，这可能与这些细胞的不同分化能力和内在特性有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b920/11833352/0f8e97493fd8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b920/11833352/bc9942239f37/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b920/11833352/447c3b6b51f3/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b920/11833352/5224dce8be61/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b920/11833352/0f8e97493fd8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b920/11833352/bc9942239f37/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b920/11833352/447c3b6b51f3/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b920/11833352/5224dce8be61/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b920/11833352/0f8e97493fd8/gr4.jpg

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J Neurosci Methods. 2023 Feb 15;386:109778. doi: 10.1016/j.jneumeth.2022.109778. Epub 2022 Dec 23.
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Spinal Cord Injury: A Systematic Review and Network Meta-Analysis of Therapeutic Strategies Based on 15 Types of Stem Cells in Animal Models.脊髓损伤：基于动物模型中15种干细胞的治疗策略的系统评价和网状Meta分析
Front Pharmacol. 2022 Mar 14;13:819861. doi: 10.3389/fphar.2022.819861. eCollection 2022.
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Chronic spinal cord injury functionally repaired by direct implantation of encapsulated hair-follicle-associated pluripotent (HAP) stem cells in a mouse model: Potential for clinical regenerative medicine.
毛乳头相关多能（HAP）干细胞囊封直接植入修复慢性脊髓损伤的功能：临床再生医学的潜力。
PLoS One. 2022 Jan 27;17(1):e0262755. doi: 10.1371/journal.pone.0262755. eCollection 2022.
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Biological Functions and Therapeutic Potential of Autophagy in Spinal Cord Injury.自噬在脊髓损伤中的生物学功能及治疗潜力
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Pursuing More Aggressive Timelines in the Surgical Treatment of Traumatic Spinal Cord Injury (TSCI): A Retrospective Cohort Study with Subgroup Analysis.在创伤性脊髓损伤（TSCI）手术治疗中追求更积极的时间线：一项带有亚组分析的回顾性队列研究。
J Clin Med. 2021 Dec 20;10(24):5977. doi: 10.3390/jcm10245977.
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