Tameling Wladimir I L
Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.
Methods Mol Biol. 2012;835:585-90. doi: 10.1007/978-1-61779-501-5_36.
Nucleotide-binding, leucine-rich repeat (NB-LRR) immune receptors from plants confer resistance to fungal pathogens and many other pathogenic organisms. Their low expression makes it challenging to purify these receptors from plants in sufficient quantities to be able to identify interacting proteins by mass spectrometry. Here we describe a protocol to affinity-purify recombinant NB-LRR immune receptors, fused to the streptavidin-binding peptide tag.
植物中的核苷酸结合富含亮氨酸重复序列（NB-LRR）免疫受体可赋予对真菌病原体和许多其他致病生物的抗性。它们的低表达使得从植物中大量纯化这些受体具有挑战性，难以通过质谱法鉴定相互作用的蛋白质。在此，我们描述了一种亲和纯化与链霉亲和素结合肽标签融合的重组NB-LRR免疫受体的方案。
