Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):28-32. doi: 10.1073/pnas.1107524108. Epub 2011 Dec 19.
We performed label-free observation of molecular dynamics in apoptotic cells by Raman microscopy. Dynamic changes in cytochrome c distribution at the Raman band of 750 cm(-1) were observed after adding an apoptosis inducer to the cells. The comparison of mitochondria fluorescence images and Raman images of cytochrome c confirmed that changes in cytochrome c distribution can be distinguished as release of cytochrome c from mitochondria. Our observation also revealed that the redox state of cytochrome c was maintained during the release from the mitochondria. Monitoring mitochondrial membrane potential with JC-1 dye confirmed that the observed cytochrome c release was associated with apoptosis.
我们通过拉曼显微镜对凋亡细胞中的分子动力学进行了无标记观察。向细胞中加入凋亡诱导剂后,观察到拉曼带 750 cm(-1)处细胞色素 c 分布的动态变化。线粒体荧光图像与细胞色素 c 的拉曼图像的比较证实,细胞色素 c 分布的变化可以区分细胞色素 c 从线粒体中的释放。我们的观察还表明,细胞色素 c 的氧化还原状态在从线粒体释放过程中得以维持。用 JC-1 染料监测线粒体膜电位证实,观察到的细胞色素 c 释放与细胞凋亡有关。