Institute for Medical Biology of the Polish Academy of Sciences, Łódź, Poland.
Pol J Microbiol. 2011;60(3):259-63.
Giardia intestinalis is a complex species divided into 7 assemblages (A - G). Two of them (A and B) are infective for both humans and animals. In cats four assemblages can occur: A, B, D, and F Assemblages A and B infect either cats, dogs and humans, assemblage D infects cats and dogs and assemblage F only cats. The purpose of this study was to determine the prevalence and genotypes of G. intestinalis in cats from Warsaw. From November 2006 to March 2007 a hundred sixty samples of stool were collected and examined by light microscopy. G. intestinalis cysts were detected in 3.75% of samples. DNA extracted from positive samples was used as template for PCR-RFLP using Giardia specific primers and the amplicons were sequenced. A comparison of the obtained DNA sequences with the Giardia sequences in the GeneBank database revealed assemblage A in 1.25% of the investigated cats, assemblage B in 1.25% and D in 1.25%.
肠道贾第虫是一个复杂的物种，分为 7 个集合（A-G）。其中两个（A 和 B）对人类和动物都具有传染性。在猫中，可能会出现四个集合：A、B、D 和 F。集合 A 和 B 感染猫、狗和人类，集合 D 感染猫和狗，而集合 F 只感染猫。本研究旨在确定华沙猫中肠道贾第虫的流行情况和基因型。2006 年 11 月至 2007 年 3 月，收集了 160 份粪便样本，并通过光学显微镜进行检查。在 3.75%的样本中检测到肠道贾第虫包囊。从阳性样本中提取的 DNA 用作聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的模板，使用肠道贾第虫特异性引物扩增，并对扩增子进行测序。将获得的 DNA 序列与 GeneBank 数据库中的肠道贾第虫序列进行比较，发现 1.25%的受调查猫中存在集合 A，1.25%的猫中存在集合 B，1.25%的猫中存在集合 D。
