Štrkolcová G, Goldová M, Maďar M, Čechová L, Halánová M, Mojžišová J
Department of Epizootiology and Parasitology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 041 81, Slovak Republic.
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 041 81, Slovak Republic.
Parasitol Res. 2016 May;115(5):1939-44. doi: 10.1007/s00436-016-4935-3. Epub 2016 Jan 28.
For an overview on the occurrence of Giardia assemblages in children in Eastern Slovakia, we examined 259 faecal samples of children from the segregated settlement in Medzev, 30 samples of children from the orphanage in Medzev and 40 samples of children with autism from the Special Elementary School in Košice. Thirty-eight samples (14.67 %) from the segregated settlement, 19 samples (63.33 %) from the orphanage and two samples (5.0 %) from the Special Elementary School were positive for Giardia by flotation. The initial microscopic diagnostics were completed by the genotyping of the triosephosphate isomerase-gene loci (tpi genes) which revealed the existence of two Giardia assemblages in Slovak population, namely Giardia duodenalis (assemblage A) and Giardia enterica (assemblage B). These results represent the first evidence of A and B assemblages in children in Slovakia. Epidemiological significance and the impact on the public health of Giardia infection are highlighted.
为了概述斯洛伐克东部儿童中贾第虫组合的出现情况，我们检测了来自梅德泽夫隔离定居点的259份儿童粪便样本、来自梅德泽夫孤儿院的30份儿童样本以及来自科希策特殊小学的40份自闭症儿童样本。通过浮选法检测发现，来自隔离定居点的38份样本（14.67%）、来自孤儿院的19份样本（63.33%）以及来自特殊小学的2份样本（5.0%）贾第虫呈阳性。最初的显微镜诊断通过磷酸丙糖异构酶基因位点（tpi基因）的基因分型完成，结果显示斯洛伐克人群中存在两种贾第虫组合，即十二指肠贾第虫（组合A）和肠道贾第虫（组合B）。这些结果是斯洛伐克儿童中A和B组合的首个证据。文中强调了贾第虫感染的流行病学意义及其对公众健康的影响。
