Bibi Yamin, Nisa Sobia, Zia Muhammad, Waheed Abdul, Ahmed Sabbir, Chaudhary M Fayyaz
Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
Pak J Pharm Sci. 2012 Jan;25(1):183-7.
Aesculus indica (Linn.) (Sapindaceae) is an ethanobotanically important plant specie traditionally used against rheumatism, skin and vein complaints. Cytotoxic potential of Aesculus indica crude leaf extract and its fractions was investigated against MCF-7 cell line. Crude extract of Aesculus indica was prepared in methanol by maceration technique. Crude extract was fractionated into four organic and one aqueous fraction on polarity basis. MTT assay was used to evaluate the reduction of viability of MCF-7 breast cancer cell line. Cell viability was inhibited by Aesculus indica crude extract in a dose dependent manner ranging from 34.2% at 10 μg/ml to 94% at 500μg/ml. Activity was found in an ascending order from hexane showing 29.8% inhibition to aqueous fraction indicating maximum inhibition, 60%. Phytochemical analysis of crude and fractionated extracts revealed presence of flavonoids, saponins, coumarins and tannins upto varying degrees. Methanol and aqueous fraction of methanol extract of Aesculus indica can be good source of cytotoxic compounds.
印度七叶树（Linn.）（无患子科）是一种在民族植物学上具有重要意义的植物物种，传统上用于治疗风湿病、皮肤和静脉疾病。研究了印度七叶树粗叶提取物及其馏分对MCF-7细胞系的细胞毒性潜力。采用浸渍技术在甲醇中制备了印度七叶树粗提取物。根据极性将粗提取物分离为四个有机馏分和一个水馏分。采用MTT法评估MCF-7乳腺癌细胞系活力的降低情况。印度七叶树粗提取物以剂量依赖性方式抑制细胞活力，浓度范围从10μg/ml时的34.2%到500μg/ml时的94%。活性呈现出从己烷的29.8%抑制到水馏分的最大抑制60%的升序排列。对粗提取物和分离提取物的植物化学分析表明存在不同程度的黄酮类化合物、皂苷、香豆素和单宁。印度七叶树甲醇提取物的甲醇和水馏分可能是细胞毒性化合物的良好来源。
