Institute of Biochemical Engineering, Technische Universität Braunschweig, Gaußstrasse 17, 38106, Braunschweig, Germany.
Biotechnol Lett. 2012 Apr;34(4):717-20. doi: 10.1007/s10529-011-0827-x. Epub 2011 Dec 21.
Violacein and deoxyviolacein display a broad range of interesting biological properties but their production is rarely distinguished due to the lack of suitable analytical methods. An HPLC method has been developed for the separation and quantification of violacein and deoxyviolacein and can determine the content of both molecules in microbial cultures. A comparison of different production microorganisms, including recombinant Escherichia coli and the natural producer Janthinobacterium lividum, revealed that the formation of violacein and deoxyviolacein is strain-specific but showed significant variation during growth although the ratio between the two compounds remained constant.
紫黑素和脱氧紫黑素具有广泛的有趣的生物学特性，但由于缺乏合适的分析方法，它们的生产很少被区分开来。已经开发出一种 HPLC 方法用于分离和定量紫黑素和脱氧紫黑素，并可以确定微生物培养物中这两种分子的含量。对不同生产微生物的比较，包括重组大肠杆菌和天然产生菌 Janthinobacterium lividum，表明紫黑素和脱氧紫黑素的形成是菌株特异性的，但在生长过程中存在显著差异，尽管两种化合物之间的比例保持不变。
