Université de Technologie de Compiègne, ESCOM, TIMR (Integrated Transformations of Renewable Matter), Centre de Recherche Royallieu-CS 60319, 60203 Compiègne CEDEX, France.
Laboratoire CTA, UR TVA, Centre d'Analyses et de Recherche, Faculté des Sciences, Université Saint-Joseph, Beyrouth 1104 2020, Lebanon.
Molecules. 2023 May 24;28(11):4292. doi: 10.3390/molecules28114292.
Violacein and deoxyviolacein are bis-indole pigments synthesized by a number of microorganisms. The present study describes the biosynthesis of a mixture of violacein and deoxyviolacein using a genetically modified strain as a production chassis, the subsequent extraction of the intracellular pigments, and ultimately their purification using column chromatography. The results show that the optimal separation between the pigments occurs using an ethyl acetate/cyclohexane mixture with different ratios, first 65:35 until both pigments were clearly visible and distinguishable, then 40:60 to create a noticeable separation between them and recover the deoxyviolacein, and finally 80:20, which allows the recovery of the violacein. The purified pigments were then analyzed by thin-layer chromatography and nuclear magnetic resonance.
紫色素和脱氧紫色素是由许多微生物合成的双吲哚色素。本研究描述了使用遗传修饰的 菌株作为生产底盘合成紫色素和脱氧紫色素混合物的过程，随后对细胞内色素进行提取，最终使用柱层析法进行纯化。结果表明，使用不同比例的乙酸乙酯/环己烷混合物可以实现最佳的色素分离，首先是 65:35，直到两种色素都清晰可见并可区分，然后是 40:60，以在它们之间形成明显的分离并回收脱氧紫色素，最后是 80:20，这允许回收紫色素。然后通过薄层层析和核磁共振分析纯化后的色素。
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