Abuaf N, Rostane H, Barbara J, Toly-Ndour C, Gaouar H, Mathelier-Fusade P, Leynadier F, Francès C, Girot R
Service d'Hématologie et d'Immunologie Biologique, Hôpital Tenon (AP-HP), Université Pierre et Marie Curie, 4 Rue de la Chine, 75020 Paris, France.
J Allergy (Cairo). 2012;2012:580873. doi: 10.1155/2012/580873. Epub 2011 Dec 1.
Background. An in vitro basophil activation test, based on the detection of CD63 upregulation induced by NSAIDs, has been described. Its clinical significance remains controversial. Objectives. In patients with a history of nonallergic NSAID hypersensitivity, stratified according to the severity of the symptoms, to assess with NSAIDs the predictive value of basophil (BAT) and monocyte (MAT) activation tests. Patients/Methods. Sixty patients who had NSAIDs-induced or exacerbated urticaria/angiooedema and 20 controls was included. After incubation with NSAIDs or acetaminophen, leukocytes were analysed for CD63 upregulation. Results. With aspirin, the sensitivity (37%) and specificity (90%) of BAT agree with already published results. In contrast, when patients had had cutaneous and visceral reactions, the frequency of positive BAT 14/22 (64%, P < 0.001) or MAT 10/22 (46%, P < 0.01) were increased. Conclusions. Positive tests were more frequent among patients having a severe hypersensitivity contrasting with the other patients who had results similar to controls.
背景。一种基于检测非甾体抗炎药（NSAIDs）诱导的CD63上调的体外嗜碱性粒细胞活化试验已被描述。其临床意义仍存在争议。目的。在有非过敏性NSAIDs超敏反应病史的患者中，根据症状严重程度分层，用NSAIDs评估嗜碱性粒细胞（BAT）和单核细胞（MAT）活化试验的预测价值。患者/方法。纳入60例有NSAIDs诱导或加重的荨麻疹/血管性水肿患者和20例对照。在与NSAIDs或对乙酰氨基酚孵育后，分析白细胞的CD63上调情况。结果。对于阿司匹林，BAT的敏感性（37%）和特异性（90%）与已发表的结果一致。相比之下，当患者有皮肤和内脏反应时，BAT阳性频率14/22（64%，P < 0.001）或MAT阳性频率10/22（46%，P < 0.01）增加。结论。与结果与对照相似的其他患者相比，严重超敏反应患者的阳性试验更为频繁。
