RNA干扰介导的α-烯醇化酶敲低增加了肿瘤细胞对抗微管蛋白化疗药物的敏感性。
RNAi-mediated knockdown of α-enolase increases the sensitivity of tumor cells to antitubulin chemotherapeutics.
作者信息
Georges Elias, Bonneau Anne-Marie, Prinos Panagiotis
出版信息
Int J Biochem Mol Biol. 2011;2(4):303-8. Epub 2011 Oct 21.
Abstract
The over-expression of α-enolase was demonstrated in several cancers, including lung, brain, breast, colon and prostate. In this report, we investigated the effects of α-enolase knockdown on the sensitivity of cancer cells to chemotherapeutic drugs. RNAi-mediated knockdown of α-enolase in A549 and H460 lung, MCF7 breast and CaOV3 ovarian cancer cells caused a significant increase in the sensitivity of these cells to antitubulin chemotherapeutics (e.g., vincristine and taxol), but not to doxorubicin, etoposide or cisplatinum. This is the first demonstration showing the effects of α-enolase expression on the sensitivity of tumor cells to clinically relevant chemotherapeutics.
摘要
α-烯醇化酶在包括肺癌、脑癌、乳腺癌、结肠癌和前列腺癌在内的多种癌症中均有过表达。在本报告中，我们研究了敲低α-烯醇化酶对癌细胞对化疗药物敏感性的影响。RNA干扰介导的A549和H460肺癌细胞、MCF7乳腺癌细胞以及CaOV3卵巢癌细胞中α-烯醇化酶的敲低，导致这些细胞对抗微管化疗药物（如长春新碱和紫杉醇）的敏感性显著增加，但对阿霉素、依托泊苷或顺铂则无此效果。这是首次证明α-烯醇化酶表达对肿瘤细胞对临床相关化疗药物敏感性的影响。
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本文引用的文献
1
Regulation of cancer cell metabolism.癌细胞代谢的调控。
Nat Rev Cancer. 2011 Feb;11(2):85-95. doi: 10.1038/nrc2981.
2
P-glycoprotein (ABCB1) modulates collateral sensitivity of a multidrug resistant cell line to verapamil.P-糖蛋白（ABCB1）调节多药耐药细胞系对维拉帕米的旁系敏感性。
Arch Biochem Biophys. 2009 Nov;491(1-2):53-60. doi: 10.1016/j.abb.2009.09.012. Epub 2009 Sep 20.
3
Multifaceted roles of glycolytic enzymes.糖酵解酶的多方面作用。
Trends Biochem Sci. 2005 Mar;30(3):142-50. doi: 10.1016/j.tibs.2005.01.005.
4
A role for survivin in chemoresistance of endothelial cells mediated by VEGF.存活素在血管内皮生长因子介导的内皮细胞化疗耐药中的作用。
Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4349-54. doi: 10.1073/pnas.072586399. Epub 2002 Mar 26.
5
Possible mechanisms of acquired resistance to anti-angiogenic drugs: implications for the use of combination therapy approaches.抗血管生成药物获得性耐药的可能机制：对联合治疗方法应用的启示
Cancer Metastasis Rev. 2001;20(1-2):79-86. doi: 10.1023/a:1013172910858.
6
Multifunctional alpha-enolase: its role in diseases.多功能α-烯醇化酶：其在疾病中的作用。
Cell Mol Life Sci. 2001 Jun;58(7):902-20. doi: 10.1007/pl00000910.
7
VEGF: an update on biological and therapeutic aspects.血管内皮生长因子：生物学与治疗学方面的最新进展
Curr Opin Biotechnol. 2000 Dec;11(6):617-24. doi: 10.1016/s0958-1669(00)00153-1.
8
The glycolytic enzyme enolase is present in sperm tail and displays nucleotide-dependent association with microtubules.
Eur J Cell Biol. 2000 Feb;79(2):104-11. doi: 10.1078/S0171-9335(04)70012-6.
9
Pyruvate kinase as a microtubule destabilizing factor in vitro.
Biochem Biophys Res Commun. 1999 Jan 19;254(2):430-5. doi: 10.1006/bbrc.1998.9957.
10
Endothelial cell hypoxic stress proteins.内皮细胞缺氧应激蛋白
J Lab Clin Med. 1998 Dec;132(6):456-63. doi: 10.1016/s0022-2143(98)90122-6.
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